GRX5 monothiol glutaredoxin GRX5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GRX5
Official Full Name: monothiol glutaredoxin GRX5
Primary source: SGD:S000005980
Locus tag: YPL059W
See related: AllianceGenome:SGD:S000005980; FungiDB:YPL059W; VEuPathDB:YPL059W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 2 iron, 2 sulfur cluster binding activity and disulfide oxidoreductase activity. Involved in cellular response to oxidative stress; protein maturation by iron-sulfur cluster transfer; and response to osmotic stress. Located in mitochondrial matrix. Human ortholog(s) of this gene implicated in autosomal recessive pyridoxine-refractory sideroblastic anemia 3. Orthologous to human GLRX5 (glutaredoxin 5). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GRX5 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (444579..445031)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Potential roles for monothiol Grx5 in scavenging and recycling linear [Fe3S4](+) clusters released during protein unfolding under oxidative stress conditions and in maturation of [Fe4S4](2+) cluster-containing proteins
Title: Monothiol glutaredoxins can bind linear [Fe3S4]+ and [Fe4S4]2+ clusters in addition to [Fe2S2]2+ clusters: spectroscopic characterization and functional implications.
Data show that mitochondrial Hsp70 chaperone Ssq1 interacts with the monothiol glutaredoxin 5 (Grx5) at a binding site different from that of Isu1.
Title: The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation.
suggest an interaction between Grx5 and SPT10 via intermolecular disulfide linkage and propose a model for a role of Grx5 in the regulation of protein expression under the control of SPT10
Title: Interaction between Saccharomyces cerevisiae glutaredoxin 5 and SPT10 and their in vivo functions.
Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5 are reported.
Title: Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5.
Grx5 acts on iron-sulfur cluster biosynthesis by regulating the redox state of important cysteine residues in Nfs1 and/or in the scaffold proteins where iron-sulfur clusters initially assemble
Title: Predictive reconstruction of the mitochondrial iron-sulfur cluster assembly metabolism. II. Role of glutaredoxin Grx5.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 2 iron, 2 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables 2 iron, 2 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables disulfide oxidoreductase activity	IDA Inferred from Direct Assay more info	PubMed
enables disulfide oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables disulfide oxidoreductase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by [2Fe-2S] cluster transfer	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by [4Fe-4S] cluster transfer	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by iron-sulfur cluster transfer	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein maturation by iron-sulfur cluster transfer	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: monothiol glutaredoxin GRX5

NP_015266.1

Glutathione-dependent oxidoreductase; mitochondrial matrix protein involved at an early step in the biogenesis of iron-sulfur centers along with Bol1p; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

444579..445031

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183873.1 → NP_015266.1 TPA: monothiol glutaredoxin GRX5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015266.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3V5, Q02784

UniProtKB/TrEMBL

A6ZWI9, B3LL09, C7GTZ7, C8ZIX1, G2WPC0, N1NVH8

Conserved Domains (1) summary

COG0278
Location:31 → 137

GrxD; Glutaredoxin-related protein [Posttranslational modification, protein turnover, chaperones]