HYP2 translation elongation factor eIF-5A [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: HYP2
Official Full Name: translation elongation factor eIF-5A
Primary source: SGD:S000000760
Locus tag: YEL034W
See related: AllianceGenome:SGD:S000000760; FungiDB:YEL034W; VEuPathDB:YEL034W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TIF51A
Summary: Enables RNA binding activity; ribosome binding activity; and translation factor activity, RNA binding. Involved in positive regulation of translation and translational frameshifting. Located in perinuclear region of cytoplasm. Used to study pancreatic ductal adenocarcinoma. Orthologous to several human genes including EIF5AL1 (eukaryotic translation initiation factor 5A like 1) and EIF5A2 (eukaryotic translation initiation factor 5A2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See HYP2 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (85676..86149)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cells.
Title: SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cells.
Hypusinated eIF5A Promotes Ribosomal Frameshifting during Decoding of ODC Antizyme mRNA in Saccharomyces cerevisiae.
Title: Hypusinated eIF5A Promotes Ribosomal Frameshifting during Decoding of ODC Antizyme mRNA in Saccharomyces cerevisiae.
The activator/repressor Hap1 binds to the yeast eIF5A-encoding gene TIF51A to adapt its expression to the mitochondrial functional status.
Title: The activator/repressor Hap1 binds to the yeast eIF5A-encoding gene TIF51A to adapt its expression to the mitochondrial functional status.
In vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide.
Title: In vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide.
The overexpression of eIF1 improved expression of HIS4(AUG) allele and GCN4 transcript causing 3AT resistance, whereas overexpression of eIF1 resulted in diminished UUG codon recognition of HIS4(UUG) allele causing 3AT sensitivity, despite having higher GCN4 expression
Title: Fidelity of HIS4 start codon selection influences 3-amino-1,2,4-triazole sensitivity in GTPase activating protein function defective eIF5.
the ability of eIF5A from tif51A-4 to bind to the ribosome while potentially blocking physical interaction with P-tRNA could explain its dominant negative phenotype.
Title: Mapping surface residues of eIF5A that are important for binding to the ribosome using alanine scanning mutagenesis.
Data show that translation elongation factor eIF-5A (eIF5A) requirement for polyproline synthesis is imposed by proline, not by tRNAPro.
Title: Amino acid substrates impose polyamine, eIF5A, or hypusine requirement for peptide synthesis.
eIF5A facilitates translation termination globally and promotes the elongation of many non polyproline-specific tripeptide sequences.
Title: eIF5A facilitates translation termination globally and promotes the elongation of many non polyproline-specific tripeptide sequences.
eIF5A strongly promotes the translation of the stalling sequences identified by profiling and increases the rate of peptidyl-tRNA hydrolysis more than 17-fold.
Title: eIF5A Functions Globally in Translation Elongation and Termination.
tRNA. These findings would support a model whereby eIF-5A stimulates peptide bond formation on polyproline-stalled ribosomes by stabilizing and orienting the CCA-end of the P-tRNA
Title: Structure of the hypusinylated eukaryotic translation factor eIF-5A bound to the ribosome.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables ribosome binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosome binding	IEA Inferred from Electronic Annotation more info
enables translation elongation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation elongation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation elongation factor activity	IEA Inferred from Electronic Annotation more info
enables translation elongation factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in positive regulation of cytoplasmic translational elongation through polyproline stretches	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translational elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of translational elongation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of translational elongation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of translational elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of translational termination	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of translational termination	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational elongation	IEA Inferred from Electronic Annotation more info
involved_in translational frameshifting	IGI Inferred from Genetic Interaction more info	PubMed
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: translation elongation factor eIF-5A

NP_010880.3

Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has paralog ANB1 that arose from whole genome duplication; in humans EIF5A variants cause a disorder characterized by variable combinations of developmental delay, microcephaly, micrognathia and dysmorphism; human EIF5A complements inviability of yeast hyp2 anb1 double null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

85676..86149

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178849.3 → NP_010880.3 TPA: translation elongation factor eIF-5A [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010880.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLL5, P23301

UniProtKB/TrEMBL

A6ZQR6, B3LRY3, C8Z6W1, G2WCJ9, N1P3S8

Conserved Domains (1) summary

cl30809
Location:1 → 153

PLN03107; eukaryotic translation initiation factor 5A; Provisional