PKH1 serine/threonine protein kinase PKH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PKH1
Official Full Name: serine/threonine protein kinase PKH1
Primary source: SGD:S000002898
Locus tag: YDR490C
See related: AllianceGenome:SGD:S000002898; FungiDB:YDR490C; VEuPathDB:YDR490C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including cell integrity MAPK cascade; late endosome to vacuole transport via multivesicular body sorting pathway; and positive regulation of cytoplasmic mRNA processing body assembly. Located in cell cortex and cytosol. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to human PDPK1 (3-phosphoinositide dependent protein kinase 1) and PDPK2P (3-phosphoinositide dependent protein kinase 2, pseudogene). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PKH1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1431968..1434268, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thus, Sch9 is a Cst6/Rca1 kinase that links CO2 adaptation to lipid signaling via Pkh1/2 in fungi.
Title: Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9.
PI kinase-mediated Endoplasmic Reticulum-Plasma Membrane cross-talk comprises a regulatory system that ensures cellular integrity under membrane stress conditions.
Title: Phosphoinositide kinase signaling controls ER-PM cross-talk.
Data indicate that mutant nce102 sng1 cells show features of impaired Pkh/Ypk signalling, including increased reactive oxygen species accumulation.
Title: Sng1 associates with Nce102 to regulate the yeast Pkh-Ypk signalling module in response to sphingolipid status.
Serine, threonine and valine activated a signaling network in which Sch9 integrates TORC1 and Pkh signaling via phosphorylation of threonines 570 and 737
Title: Serine- and threonine/valine-dependent activation of PDK and Tor orthologs converge on Sch9 to promote aging.
These results suggest that sphingolipid signalling through Pkh1p-Sch9p mediate mitochondrial dysfunction, oxidative stress sensitivity and shortened chronological lifespan in the yeast model of Niemann-Pick type C disease.
Title: Sphingolipid signalling mediates mitochondrial dysfunctions and reduced chronological lifespan in the yeast model of Niemann-Pick type C1.
Vps27 phosphorylation by Pkh1/2 kinases regulates the coordinated cascade of endosomal sorting complex required for transport complex recruitment at the endosomal membrane.
Title: Pkh1/2-dependent phosphorylation of Vps27 regulates ESCRT-I recruitment to endosomes.
Pkh1 protein kinase contributes to the divergent regulation of protein kinase A catalytic subunits.
Title: The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae.
Data show a mechanism through which the conserved Pkh-Ypk kinase cascade and Cdc55-PP2A facilitate rapid, transient sphingolipid production in response to heat stress through Orm protein phosphoregulation.
Title: Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways.
the interaction between Pkh1 and Gcn2 was confirmed by co-immunoprecipitation and Pkh1 was shonwn to phosphorylate Gcn2 in vitro.
Title: Pkh1 interacts with and phosphorylates components of the yeast Gcn2/eIF2α system.
Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast
Title: Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell integrity MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell integrity MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cytoplasmic mRNA processing body assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of nuclear-transcribed mRNA poly(A) tail shortening	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine/threonine protein kinase PKH1

NP_010778.3

EC 2.7.11.1

Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ypk2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1431968..1434268 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180798.3 → NP_010778.3 TPA: serine/threonine protein kinase PKH1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010778.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTB3, Q03407

UniProtKB/TrEMBL

C7GKS8, C8Z648, N1P5S2

Conserved Domains (2) summary

smart00220
Location:125 → 391

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05581
Location:123 → 391

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1