STN1 Stn1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STN1
Official Full Name: Stn1p
Primary source: SGD:S000002489
Locus tag: YDR082W
See related: AllianceGenome:SGD:S000002489; FungiDB:YDR082W; VEuPathDB:YDR082W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables single-stranded telomeric DNA binding activity and translation elongation factor binding activity. Involved in negative regulation of telomere maintenance via telomerase and telomere capping. Part of CST complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See STN1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (610441..611925)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Telomere length regulation by Rif1 protein from Hansenula polymorpha.
Title: Telomere length regulation by Rif1 protein from Hansenula polymorpha.
Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions.
Title: Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions.
Ten1, Cdc13 and Stn1, were found to physically associate with Spt5. This study proposes that Spt5 represents the target of the Cdc13-Stn1-Ten1 complex in transcription regulation.
Title: The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription.
We have shown that levels of Stn1 are dramatically reduced by upstream overlapping open reading frame (oORFs). In yeast, STN1 contains both a upstream ORF and an oORF but the latter is a far more potent inhibitor of gene expression. The STN1 oORF reduces gene expression by at least two routes.
Title: Overlapping open reading frames strongly reduce human and yeast STN1 gene expression and affect telomere function.
Data support a model that Stn1 and Ten1 can function in a Cdc13-independent manner and have implications for the function of CST components across eukaryotes.
Title: Interplay between nonsense-mediated mRNA decay and DNA damage response pathways reveals that Stn1 and Ten1 are the key CST telomere-cap components.
Data show that by controlling the timing of Cdc13 and Stn1 phosphorylations, Cdk1 regulates the temporal recruitment of telomerase complexes and Cdc13-Stn1-Ten1 (CST) complexes to the telomeres to facilitate telomere maintenance.
Title: Cdk1 regulates the temporal recruitment of telomerase and Cdc13-Stn1-Ten1 complex for telomere replication.
Dimerization of the OB2 domain of Cdc13 is required for proper Cdc13, Stn1, Ten1 (CST) assembly and productive telomere capping.
Title: Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance.
the crystal structures of the C-terminal domain of the Saccharomyces cerevisiae Stn1 and the Schizosaccharomyces pombe Ten1 proteins are reported.
Title: Telomere capping proteins are structurally related to RPA with an additional telomere-specific domain.
Stn1 overrides DNA replication control through the S phase checkpoint
Title: Yeast telomere capping protein Stn1 overrides DNA replication control through the S phase checkpoint.
The N terminus of Stn1 interacts with Ten1 and carries out its essential capping function. The C terminus of Stn1 binds both Cdc13 and Pol12.
Title: Distinct roles for yeast Stn1 in telomere capping and telomerase inhibition.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables single-stranded telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables translation elongation factor binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of telomere maintenance via telomerase	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere capping	IEA Inferred from Electronic Annotation more info
involved_in telomere capping	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere capping	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of CST complex	IEA Inferred from Electronic Annotation more info
part_of CST complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Stn1p

NP_010367.1

Telomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

610441..611925

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180390.1 → NP_010367.1 TPA: Stn1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010367.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VS69, P38960

UniProtKB/TrEMBL

C7GVQ4, C8Z500, N1P6J2

Conserved Domains (2) summary

pfam10451
Location:11 → 261

Stn1; Telomere regulation protein Stn1

pfam12659
Location:363 → 489

Stn1_C; Telomere capping C-terminal wHTH