DIA2 DNA-binding SCF ubiquitin ligase subunit DIA2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DIA2
Official Full Name: DNA-binding SCF ubiquitin ligase subunit DIA2
Primary source: SGD:S000005606
Locus tag: YOR080W
See related: AllianceGenome:SGD:S000005606; FungiDB:YOR080W; VEuPathDB:YOR080W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YOR29-31
Summary: Enables DNA replication origin binding activity. Contributes to ubiquitin-protein transferase activity. Involved in several processes, including SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; constitutive heterochromatin formation; and invasive growth in response to glucose limitation. Located in nuclear replication fork. Part of SCF ubiquitin ligase complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DIA2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (474594..476792)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here, using a panel of reporter assays, we demonstrate that mating pheromone stimulates vacuolar targeting of a cytoplasmic reporter protein and that this process depends on Vps34. Through a systematic analysis of F-box deletion mutants, we show that Dia2 is required to sustain pheromone-induced vacuolar targeting
Title: Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.
Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1.
Title: Degradation of Mrc1 promotes recombination-mediated restart of stalled replication forks.
The tethering of SCF(Dia2) to the replisome progression complex increases the efficiency of ubiquitylation of the Mcm7 subunit of CMG. Correspondingly, loss of tethering is synthetic lethal in combination with a disassembly-defective allele of CDC48.
Title: Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase.
the ubiquitin ligase known as SCF(Dia2) promotes ubiquitylation of CMG helicase during the final stages of chromosome replication in Saccharomyces cerevisiae.
Title: Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication.
Dia2 mediates Mrc1 degradation to help cells resume the cell cycle during recovery from MMS-induced DNA damage in S-phase.
Title: The Saccharomyces cerevisiae F-box protein Dia2 is a mediator of S-phase checkpoint recovery from DNA damage.
Tom1 and the Dia2 control Cdc6 degradation in G1 phase.
Title: The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase.
Dia2 is required for the regulation of Sir4 binding to chromatin during S phase of the cell cycle
Title: The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing.
Dia2, by controlling the RSC chromatin remodeller, fine-tunes transcription by controlling nucleosome positioning during transcriptional activation and repression.
Title: Dia2 controls transcription by mediating assembly of the RSC complex.
The intra-S phase checkpoint protein Tof1 collaborates with the helicase Rrm3 and the F-box protein Dia2 to maintain genome stability in Saccharomyces cerevisiae.
Title: The intra-S phase checkpoint protein Tof1 collaborates with the helicase Rrm3 and the F-box protein Dia2 to maintain genome stability in Saccharomyces cerevisiae.
Dia2 protein turnover does not involve an autocatalytic mechanism and that Dia2 proteolysis is inhibited by activation of the replication stress response.
Title: Activation of the S-phase checkpoint inhibits degradation of the F-box protein Dia2.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in invasive growth in response to glucose limitation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic DNA replication termination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex disassembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein-containing complex disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding SCF ubiquitin ligase subunit DIA2

NP_014723.2

Origin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitination of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

474594..476792

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183499.1 → NP_014723.2 TPA: DNA-binding SCF ubiquitin ligase subunit DIA2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014723.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2E3, O00034, Q08496, Q7LGN4

UniProtKB/TrEMBL

G2WMV8

Conserved Domains (4) summary

sd00033
Location:483 → 511

LRR_RI; leucine-rich repeat [structural motif]

sd00034
Location:553 → 581

LRR_AMN1; leucine-rich repeat [structural motif]

sd00006
Location:19 → 43

TPR; TPR repeat [structural motif]

pfam00646
Location:209 → 241

F-box; F-box domain