Cryaa crystallin, alpha A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cryaaprovided by RGD
Official Full Name: crystallin, alpha Aprovided by RGD
Primary source: RGD:2413
See related: EnsemblRapid:ENSRNOG00000047175 AllianceGenome:RGD:2413
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Crya1; Acry-1
Summary: This gene encodes subunit a, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Expression: Biased expression in Spleen (RPKM 26.6) and Thymus (RPKM 5.1) See more
Orthologs: human mouse all
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Genomic context

Location:: 20p12

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	20	NC_086038.1 (9784872..9788656)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	20	NC_051355.1 (9783605..9787351)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	20	NC_005119.4 (10438444..10442189)

Chromosome 20 - NC_086038.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rat crystallin alpha A a encode activated chaperones.
Title: Species-Specific Structural and Functional Divergence of α-Crystallins: Zebrafish αBa- and Rodent αA(ins)-Crystallin Encode Activated Chaperones.
Cryaa is a moonlighting protein that functions as a heat shock protein as well as a lens crystalline.
Title: Phosphorylation of alpha-crystallin in rat lenses is stimulated by H2O2 but phosphorylation has no effect on chaperone activity.
These findings indicated that alpha-crystallin positively regulated the activation of PI3K/Akt/mTOR signaling pathway and promote the proliferation and survival of cultured olfactory ensheathing cells
Title: Alpha-crystallin promotes rat olfactory ensheathing cells survival and proliferation through regulation of PI3K/Akt/mTOR signaling pathways.
The alphaA- and alphaB-crystallin peptides inhibited stress-induced aggregation of four client proteins, and the alphaA-acetyl peptide was more effective than the native peptide against three of the client proteins.
Title: Chaperone peptides of α-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts.
alphaA-crystallin and alphaB-crystallin reside in separate subcellular compartments in the developing ocular lens
Title: αA-crystallin and αB-crystallin reside in separate subcellular compartments in the developing ocular lens.
The main differences, which could be attributed to the cataract-specific processes, are the faster decay of the content of gamma-crystallins and the significant decrease of unmodified alphaA-crystallin abundance in the senescent rat lenses.
Title: Age-related changes in the water-soluble lens protein composition of Wistar and accelerated-senescence OXYS rats.
Curcumin suppressed the expression of selenite-induced alphaA- and alphaB-crystallin and Hsp 70, and may therefore suppress cataract formation in rat pups
Title: Effect of curcumin on the modulation of αA- and αB-crystallin and heat shock protein 70 in selenium-induced cataractogenesis in Wistar rat pups.
hydroxylation and subsequent phosphorylation of a proline residue in alpha-crystallin A in the eye as well as in heart tissue of rat.
Title: Characterization of O-phosphohydroxyproline in rat {alpha}-crystallin A.
Enhanced survival of axotomized axons was observed beyond the crush site after a single intravitreal administration of alpha-crystallin at the time of axotomy.
Title: Alpha-crystallin protected axons from optic nerve degeneration after crushing in rats.
alpha A-K11T and alpha B-K90T/K92T mutants showed the largest decrease in glycation and increase in chaperone activity.
Title: Role of the specifically targeted lysine residues in the glycation dependent loss of chaperone activity of alpha A- and alpha B-crystallins.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:259595 (e-PCR)
- UniSTS:259595

Cryaa (e-PCR), detects polymorphism
- UniSTS:259595

RH94619 (e-PCR)
- UniSTS:119867

Cryaa (e-PCR), detects polymorphism
- UniSTS:231399

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables structural constituent of eye lens	TAS Traceable Author Statement more info	PubMed
enables structural molecule activity	ISO Inferred from Sequence Orthology more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin filament organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process involved in morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within camera-type eye development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within embryonic camera-type eye morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in lens development in camera-type eye	IBA Inferred from Biological aspect of Ancestor more info
involved_in lens development in camera-type eye	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within lens development in camera-type eye	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lens fiber cell morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lens morphogenesis in camera-type eye	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within microtubule-based process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mitochondrion organization	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of intracellular transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein folding	IDA Inferred from Direct Assay more info	PubMed
involved_in protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to UV-A	ISO Inferred from Sequence Orthology more info
involved_in response to glucocorticoid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to heat	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hydrogen peroxide	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in response to lead ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within tubulin complex assembly	ISO Inferred from Sequence Orthology more info
involved_in visual perception	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: alpha-crystallin A chain

Names: Crystallin, alpha polypeptide A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001289737.2 → NP_001276666.1 alpha-crystallin A chain isoform 2

See identical proteins and their annotated locations for NP_001276666.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) includes an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2, also known as alpha Ains-crystallin) is longer, compared to isoform 1.

Source sequence(s)

JAXUCZ010000020

UniProtKB/Swiss-Prot

P02496, P24623

UniProtKB/TrEMBL

A6JK11

Conserved Domains (2) summary

pfam00525
Location:1 → 51

Crystallin; Alpha crystallin A chain, N terminal

cl00175
Location:86 → 168

alpha-crystallin-Hsps_p23-like; alpha-crystallin domain (ACD) found in alpha-crystallin-type small heat shock proteins, and a similar domain found in p23 (a cochaperone for Hsp90) and in other p23-like proteins.
NM_012534.5 → NP_036666.2 alpha-crystallin A chain isoform 1

See identical proteins and their annotated locations for NP_036666.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the shorter isoform (1).

Source sequence(s)

JAXUCZ010000020

UniProtKB/TrEMBL

A0JN13

Related

ENSRNOP00000108761.1, ENSRNOT00000150532.1

Conserved Domains (2) summary

cd06497
Location:60 → 145

ACD_alphaA-crystallin_HspB4; Alpha-crystallin domain found in the small heat shock protein (sHsp) alphaA-crystallin (HspB4, 20kDa). sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, and are generally active as large oligomers ...

pfam00525
Location:1 → 51

Crystallin; Alpha crystallin A chain, N terminal