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    Brf1 BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit [ Mus musculus (house mouse) ]

    Gene ID: 72308, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Brf1provided by MGI
    Official Full Name
    BRF1, RNA polymerase III transcription initiation factor 90 kDa subunitprovided by MGI
    Primary source
    MGI:MGI:1919558
    See related
    Ensembl:ENSMUSG00000011158 AllianceGenome:MGI:1919558
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    GTF3B; TAF3C; TAFIII90; TFIIIB90; mTFIIIB90; 2510002F24Rik
    Summary
    Predicted to enable RNA polymerase III general transcription initiation factor activity and RNA polymerase III type 3 promoter sequence-specific DNA binding activity. Acts upstream of or within regulation of mRNA stability. Predicted to be part of transcription factor TFIIIB complex and transcription preinitiation complex. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in cerebellofaciodental syndrome. Orthologous to human BRF1 (BRF1 RNA polymerase III transcription initiation factor subunit). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in thymus adult (RPKM 20.5), ovary adult (RPKM 14.2) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Brf1 in Genome Data Viewer
    Location:
    12 F1; 12 61.47 cM
    Exon count:
    22
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (112919251..112964327, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (112955631..113000706, complement)

    Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene jagged 2 Neighboring gene microRNA 6941 Neighboring gene STARR-positive B cell enhancer ABC_E11526 Neighboring gene STARR-seq mESC enhancer starr_33372 Neighboring gene nudix hydrolase 14 Neighboring gene BTB domain containing 6 Neighboring gene RIKEN cDNA 9230104M06 gene Neighboring gene STARR-positive B cell enhancer ABC_E1866 Neighboring gene phosphofurin acidic cluster sorting protein 2 Neighboring gene CapStarr-seq enhancer MGSCv37_chr12:114285178-114285287 Neighboring gene CapStarr-seq enhancer MGSCv37_chr12:114285299-114285486

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas. Liko D, et al. Cell Death Differ, 2019 Dec. PMID 30858608, Free PMC Article
    2. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. Zhong S, et al. J Biol Chem, 2011 Jan 28. PMID 21106530, Free PMC Article
    3. Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference. Raineri I, et al. Nucleic Acids Res, 2004. PMID 14976220, Free PMC Article
    4. BRF1 protein turnover and mRNA decay activity are regulated by protein kinase B at the same phosphorylation sites. Benjamin D, et al. Mol Cell Biol, 2006 Dec. PMID 17030608, Free PMC Article
    5. Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase. Tan FE, et al. Proc Natl Acad Sci U S A, 2014 Apr 29. PMID 24733888, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Brf1 (zfp36l1), an AU-rich element mRNA-binding protein from the Zfp36 protein family, operates downstream of FGF/Erk MAP kinase signaling to regulate pluripotency and cell fate decision making in mouse embryonic stem cells.
      Title: Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.
    2. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression.
      Title: Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression.
    3. Phosphorylation at serine-92 and serine-203 is involved in BRF1 protein stability; high BRF1 protein levels are required to reinstate decay upon dissipation of the stabilizing signal.
      Title: BRF1 protein turnover and mRNA decay activity are regulated by protein kinase B at the same phosphorylation sites.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 
    • Targeted (4)  1 citation

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables RNA polymerase III general transcription initiation factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase III type 3 promoter sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables TBP-class protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA-templated transcription initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of transcription by RNA polymerase III ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within regulation of mRNA stability IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transcription by RNA polymerase III IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transcription preinitiation complex assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of transcription factor TFIIIB complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of transcription preinitiation complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    transcription factor IIIB 90 kDa subunit
    Names
    B-related factor 1
    BRF-1
    BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB
    hTFIIIB90

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_028193.3 → NP_082469.2  transcription factor IIIB 90 kDa subunit

      See identical proteins and their annotated locations for NP_082469.2

      Status: VALIDATED

      Source sequence(s)
      AC073562, AC160929
      Consensus CDS
      CCDS49195.1
      UniProtKB/Swiss-Prot
      Q8CFK2
      UniProtKB/TrEMBL
      G3X8S2
      Related
      ENSMUSP00000011302.8, ENSMUST00000011302.9
      Conserved Domains (2) summary
      COG1405
      Location:4 → 266
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:449 → 546
      BRF1; Brf1-like TBP-binding domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000078.7 Reference GRCm39 C57BL/6J

      Range
      112919251..112964327 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006516261.5 → XP_006516324.1  transcription factor IIIB 90 kDa subunit isoform X2

      UniProtKB/Swiss-Prot
      Q8CFK2
      Conserved Domains (2) summary
      COG1405
      Location:4 → 266
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:448 → 545
      BRF1; Brf1-like TBP-binding domain

    2. XM_006516260.5 → XP_006516323.1  transcription factor IIIB 90 kDa subunit isoform X1

      See identical proteins and their annotated locations for XP_006516323.1

      UniProtKB/Swiss-Prot
      Q8CFK2
      UniProtKB/TrEMBL
      G3X8S2
      Conserved Domains (2) summary
      COG1405
      Location:4 → 266
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:449 → 546
      BRF1; Brf1-like TBP-binding domain

    3. XM_030246945.2 → XP_030102805.1  transcription factor IIIB 90 kDa subunit isoform X6

      Conserved Domains (2) summary
      COG1405
      Location:1 → 62
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:244 → 341
      BRF1; Brf1-like TBP-binding domain

    4. XM_011244182.4 → XP_011242484.1  transcription factor IIIB 90 kDa subunit isoform X3

      See identical proteins and their annotated locations for XP_011242484.1

      Conserved Domains (2) summary
      COG1405
      Location:24 → 132
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:315 → 412
      BRF1; Brf1-like TBP-binding domain

    5. XM_030246944.2 → XP_030102804.1  transcription factor IIIB 90 kDa subunit isoform X5

      Conserved Domains (2) summary
      COG1405
      Location:1 → 62
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:245 → 342
      BRF1; Brf1-like TBP-binding domain

    6. XM_030246942.2 → XP_030102802.1  transcription factor IIIB 90 kDa subunit isoform X4

      Conserved Domains (2) summary
      COG1405
      Location:1 → 119
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:302 → 399
      BRF1; Brf1-like TBP-binding domain

    7. XM_011244185.4 → XP_011242487.1  transcription factor IIIB 90 kDa subunit isoform X5

      See identical proteins and their annotated locations for XP_011242487.1

      Conserved Domains (2) summary
      COG1405
      Location:1 → 62
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:245 → 342
      BRF1; Brf1-like TBP-binding domain

    8. XM_006516267.3 → XP_006516330.1  transcription factor IIIB 90 kDa subunit isoform X5

      See identical proteins and their annotated locations for XP_006516330.1

      Conserved Domains (2) summary
      COG1405
      Location:1 → 62
      SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
      pfam07741
      Location:245 → 342
      BRF1; Brf1-like TBP-binding domain

    RNA

    1. XR_872720.4 RNA Sequence

    2. XR_381545.5 RNA Sequence

    3. XR_001780489.3 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8CFK2.1 GenPept UniProtKB/Swiss-Prot:Q8CFK2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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