KAR3 Kar3p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: KAR3
Official Full Name: Kar3p
Primary source: SGD:S000006345
Locus tag: YPR141C
See related: AllianceGenome:SGD:S000006345; FungiDB:YPR141C; VEuPathDB:YPR141C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: OSR11
Summary: Enables microtubule binding activity and minus-end-directed microtubule motor activity. Involved in several processes, including karyogamy involved in conjugation with cellular fusion; nuclear migration involved in conjugation with cellular fusion; and regulation of mitotic spindle organization. Located in cytoplasmic microtubule and spindle pole body. Orthologous to human KIFC1 (kinesin family member C1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See KAR3 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (815734..817923, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
Title: The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
The kinesin-14 motor protein complex (Cik1-Kar3) cooperates with chromatin remodellers to mediate interactions between subtelomeric double-strand breaks and the Nup84 nuclear pore complex to ensure cell survival via break-induced replication.
Title: Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.
findings provide an example for a non-conventional translocation mechanism and can explain how Kar3 substitutes for key functions of Dynein in the yeast nucleus
Title: Non-catalytic motor domains enable processive movement and functional diversification of the kinesin-14 Kar3.
Cryoelectron microscopy generated 3-D structure of Kar3Cik1 complexed to microtubules in various nucleotide states.
Title: Common mechanistic themes for the powerstroke of kinesin-14 motors.
Kar3, with the help of its light chain, Cik1, is anchored during mating to the SPB component Spc72 that also serves as a nucleator and anchor for microtubules via their minus ends.
Title: Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.
Neck rotation and neck mimic docking in Vik1 and Cik1 is a structural mechanism for communication with Kar3.
Title: Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.
Rapid ATP binding to Kar3 is associated with rotation of the coiled-coil stalk, and the postpowerstroke ATP hydrolysis is independent of Vik1.
Title: The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.
show through structural determination of a C-terminal heterodimeric Kar3Vik1, electron microscopy, equilibrium binding, and motility that at the start of the cycle, Kar3Vik1 binds to or occludes two alphabeta-tubulin subunits on adjacent protofilaments
Title: Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.
we found that Cik1 mediates the association of Kar3 with kinetochores and this association only occurs before chromosome bipolar attachment.
Title: Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint.
a model in which Kar3Cik1 interacts with the MT lattice through an alternating cycle of Cik1 MT collision followed by Kar3 MT binding with head-head communication between Kar3 and Cik1 modulated by the Kar3 nucleotide state and intramolecular strain
Title: Kinesin Kar3Cik1 ATPase pathway for microtubule cross-linking.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables minus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in karyogamy	IEA Inferred from Electronic Annotation more info
involved_in karyogamy involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in microtubule-based process	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule	HDA more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle pole	HDA more info	PubMed
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole body	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Kar3p

NP_015467.1

EC 5.6.1.4

Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

815734..817923 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184238.1 → NP_015467.1 TPA: Kar3p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015467.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4D9, P17119

UniProtKB/TrEMBL

A6ZX18, B3LKA2, C8ZJG4, G2WPV5, N1NXT6

Conserved Domains (2) summary

COG1196
Location:98 → 387

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01366
Location:384 → 725

KISc_C_terminal; Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins