CDC4 SCF ubiquitin ligase complex subunit CDC4 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: CDC4
Official Full Name: SCF ubiquitin ligase complex subunit CDC4
Primary source: SGD:S000001885
Locus tag: YFL009W
See related: AllianceGenome:SGD:S000001885; FungiDB:YFL009W; VEuPathDB:YFL009W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphoserine residue binding activity and ubiquitin binding activity. Contributes to ubiquitin protein ligase activity. Involved in several processes, including SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle phase transition; and protein ubiquitination. Located in nuclear matrix. Part of nuclear SCF ubiquitin ligase complex. Human ortholog(s) of this gene implicated in developmental delay, hypotonia, and impaired language. Orthologous to human FBXW7 (F-box and WD repeat domain containing 7). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See CDC4 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (116145..118484)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cdc4 phospho-degrons allow differential regulation of Ame1(CENP-U) protein stability across the cell cycle.
Title: Cdc4 phospho-degrons allow differential regulation of Ame1(CENP-U) protein stability across the cell cycle.
Redundant targeting of Isr1 by two CDKs in mitotic cells.
Title: Redundant targeting of Isr1 by two CDKs in mitotic cells.
Study revealed that two essential Skp, Cullin, F-box (SCF) ubiquitin ligases defined by the F-box proteins Met30 and Cdc4 cooperatively regulate Cse4 (S. cerevisiae homolog of human centromere protein-A (CENP-A)) proteolysis under normal physiological conditions. Results suggest SCF-Met30/Cdc4-mediated proteolysis of Cse4 is one of the major mechanisms that prevents stable maintenance of Cse4 at non-centromeric regions.
Title: Skp, Cullin, F-box (SCF)-Met30 and SCF-Cdc4-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A for Chromosomal Stability in Budding Yeast.
An allosteric conduit facilitates dynamic multisite substrate recognition by the SCF ubiquitin ligase.
Title: An allosteric conduit facilitates dynamic multisite substrate recognition by the SCF(Cdc4) ubiquitin ligase.
Our observations suggest that both APC(Cdh1) and SCF(Cdc4)-dependent proteolysis of Clb6 at the G1/S border are crucial for multiple cell cycle regulated events including proper expression of Clb2, the G1/S and G2/M cell cycle transitions and for proper completion of cell division at mitotic exit.
Title: The anaphase-promoting complex works together with the SCF complex for proteolysis of the S-phase cyclin Clb6 during the transition from G1 to S phase.
an intricate coordination between Hst3 synthesis, genome-wide H3K56 deacetylation by Hst3, and cell cycle-regulated degradation of Hst3 by cyclin-dependent kinases and SCF(Cdc4).
Title: Regulation of the histone deacetylase Hst3 by cyclin-dependent kinases and the ubiquitin ligase SCFCdc4.
we show that Cln3 is redundantly targeted by two F-box proteins with key cell cycle-regulatory roles, Cdc4 and Grr1
Title: F-box protein specificity for g1 cyclins is dictated by subcellular localization.
Individually weak dispersed Sic1 phospho sites engage Cdc4 in a dynamic equilibrium. The multisite nature of this interaction confers cooperative dependence on kinase activity for Sic1 recognition
Title: Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase.
analysis of structure/function of a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase
Title: Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase.
Data show that high-affinity binding of recombinant Cdc4 to Tec1 phosphopeptides requires phosphorylation of not only T273 but also a second site, T276.
Title: Multisite phosphorylation of the Saccharomyces cerevisiae filamentous growth regulator Tec1 is required for its recognition by the E3 ubiquitin ligase adaptor Cdc4 and its subsequent destruction in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables U3 snoRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoserine residue binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G2/M transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IBA Inferred from Biological aspect of Ancestor more info
involved_in endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in sporulation resulting in formation of a cellular spore	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of 90S preribosome	IBA Inferred from Biological aspect of Ancestor more info
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SCF ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of nuclear SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: SCF ubiquitin ligase complex subunit CDC4

NP_116585.1

F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

116145..118484

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179957.1 → NP_116585.1 TPA: SCF ubiquitin ligase complex subunit CDC4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116585.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTM1, P07834

UniProtKB/TrEMBL

A7A230, B3LUK1, C8Z7S8, G2WDD2

Conserved Domains (4) summary

cd00200
Location:374 → 693

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:385 → 420

7WD40; WD40 repeat [structural motif]

pfam12937
Location:275 → 319

F-box-like

pfam16856
Location:228 → 272

CDC4_D; Cell division control protein 4 dimerization domain