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    asnS asparagine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945555, updated on 17-Dec-2024

    Summary

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    Official Symbol
    asnS
    Official Full Name
    asparagine--tRNA ligase
    Primary source
    ECOCYC:EG10094
    Locus tag
    b0930
    See related
    ASAP:ABE-0003162
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0921; lcs; tss
    Summary
    Asparaginyl-tRNA synthetase (AsnRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10094].
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    Genomic context

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    Sequence:
    NC_000913.3 (987585..988985, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene aspartate aminotransferase Neighboring gene outer membrane porin F Neighboring gene nicotinate phosphoribosyltransferase Neighboring gene aminopeptidase N

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Identification of a temperature-sensitive asparaginyl-transfer ribonucleic acid synthetase mutant of Escherichia coli. Yamamoto M, et al. J Bacteriol, 1977 Oct. PMID 334719, Free PMC Article
    2. The asparaginyl-tRNA synthetase gene encodes one of the complementing factors for thermosensitive translation in the Escherichia coli mutant strain, N4316. Aoki H, et al. Eur J Biochem, 1992 Oct 15. PMID 1425658
    3. Asparaginyl-tRNA synthetase from the Escherichia coli temperature-sensitive strain HO202. A proline replacement in motif 2 is responsible for a large increase in Km for asparagine and ATP. Madern D, et al. FEBS Lett, 1992 Mar 24. PMID 1544480
    4. Tyr-426 of the Escherichia coli asparaginyl-tRNA synthetase, an amino acid in a C-terminal conserved motif, is involved in ATP binding. Anselme J, et al. FEBS Lett, 1991 Mar 11. PMID 2009959
    5. Regulation of gene expression at the translational level. The rescue factor reverses thermosensitive protein synthesis in N4316, a conditionally-lethal mutant of Escherichia coli defective in translation. Ganoza MC, et al. Eur J Biochem, 1989 Jan 2. PMID 2643515

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables aminoacyl-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables asparagine-tRNA ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables asparagine-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables asparagine-tRNA ligase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables catalytic activity, acting on a nucleic acid IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in asparaginyl-tRNA aminoacylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within asparaginyl-tRNA aminoacylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in asparaginyl-tRNA aminoacylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within asparaginyl-tRNA aminoacylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within tRNA aminoacylation for protein translation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    asparagine--tRNA ligase
    NP_415450.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      987585..988985 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415450.1 asparagine--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415450.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A0H2YXD6, A0A1X3I8V4, A0A1X3J352, A0A1X3JJS0
      Conserved Domains (1) summary
      PRK03932
      Location:1 → 466
      asnC; asparaginyl-tRNA synthetase; Validated
    Nucleotide Protein
    Heading Accession and Version

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