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    glyS glycine--tRNA ligase subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948080, updated on 3-Dec-2024

    Summary

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    Official Symbol
    glyS
    Official Full Name
    glycine--tRNA ligase subunit beta
    Primary source
    ECOCYC:EG10410
    Locus tag
    b3559
    See related
    ASAP:ABE-0011624
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3547
    Summary
    glyS is an essential gene; the glyS mutants in the Keio collection have a second intact copy of glyS (R. [More information is available at EcoGene: EG10410]. A subunit cysteine thiol is not required for catalytic activity; however, C395 can be alkylated by NEM, resulting in loss of GlyRS activity . [More information is available at EcoCyc: EG10410].
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    Genomic context

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    Sequence:
    NC_000913.3 (3722328..3724397, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene insertion element IS150 protein InsA Neighboring gene IS150 family conserved protein InsB Neighboring gene pseudo Neighboring gene glycine--tRNA ligase subunit alpha Neighboring gene putative lipoprotein YsaB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Deletions in the large (beta) subunit of a hetero-oligomeric aminoacyl-tRNA synthetase. Toth MJ, et al. J Biol Chem, 1990 Jan 15. PMID 2404005
    2. A sulfhydryl presumed essential is not required for catalysis by an aminoacyl-tRNA synthetase. Profy AT, et al. J Biol Chem, 1986 Nov 25. PMID 3536904
    3. Rapid determination of nucleotides that define tRNA(Gly) acceptor identity. McClain WH, et al. Proc Natl Acad Sci U S A, 1991 Jul 15. PMID 2068095, Free PMC Article
    4. Internal structural features of E. coli glycyl-tRNA synthetase examined by subunit polypeptide chain fusions. Toth MJ, et al. J Biol Chem, 1986 May 25. PMID 3009467
    5. Comparative X-ray structure analysis of human and Escherichia coli tRNA(Gly) acceptor stem microhelices. Förster C, et al. Biochem Biophys Res Commun, 2008 Apr 18. PMID 18275849

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. we present a comparison between the crystal structures of the eubacterial Escherichia coli and the human tRNA(Gly) acceptor stem microhelices and their surrounding hydration patterns.
      Title: Comparative X-ray structure analysis of human and Escherichia coli tRNA(Gly) acceptor stem microhelices.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables aminoacyl-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables arginine-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to glycine-tRNA ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glycine-tRNA ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in arginyl-tRNA aminoacylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glycyl-tRNA aminoacylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in glycyl-tRNA aminoacylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of glycine-tRNA ligase complex EXP
    Inferred from Experiment
    more info
    		 PubMed 
    part_of glycine-tRNA ligase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of glycine-tRNA ligase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    glycine--tRNA ligase subunit beta
    NP_418016.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3722328..3724397 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418016.1 glycine--tRNA ligase subunit beta [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418016.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A775UUM8
      Conserved Domains (1) summary
      TIGR00211
      Location:1 → 689
      glyS; glycyl-tRNA synthetase, tetrameric type, beta subunit
    Nucleotide Protein
    Heading Accession and Version

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