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    trpB tryptophan synthase subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945768, updated on 3-Dec-2024

    Summary

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    Official Symbol
    trpB
    Official Full Name
    tryptophan synthase subunit beta
    Primary source
    ECOCYC:EG11025
    Locus tag
    b1261
    See related
    ASAP:ABE-0004234
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1255
    Summary
    otherPDB (S. typhimurium): [More information is available at EcoGene: EG11025]. The TrpB polypeptide functions as the subunit of the tetrameric (2-2) tryptophan synthase complex. [More information is available at EcoCyc: EG11025].
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    Genomic context

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    Sequence:
    NC_000913.3 (1317222..1318415, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF892 domain-containing protein YciF Neighboring gene stress-induced bacterial acidophilic repeat motifs-containing protein YciG Neighboring gene tryptophan synthase subunit alpha Neighboring gene fused indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase Neighboring gene anthranilate synthase subunit TrpD Neighboring gene anthranilate synthase subunit TrpE

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Large conformational changes in the Escherichia coli tryptophan synthase beta(2) subunit upon pyridoxal 5'-phosphate binding. Nishio K, et al. FEBS J, 2010 May. PMID 20370823
    2. Affinity labeling of the pyridoxal phosphate binding site of the beta2 subunit of Escherichia coli tryptophan synthase. Higgins W, et al. J Biol Chem, 1978 Jul 10. PMID 350880
    3. Assignment of the ends of the beta-chain of E. coli tryptophan synthase to the F1 and F2 domains. Crawford IP, et al. Biochem Biophys Res Commun, 1978 Nov 14. PMID 369556
    4. Cooperative and noncooperative binding of pyridoxal 5'-phosphate to tryptophan synthase from Escherichia coli. Bartholmes P, et al. Biochemistry, 1976 Oct 19. PMID 788781
    5. Genetic and biochemical characterization of the trpB8 mutation of Escherichia coli tryptophan synthase. An amino acid switch at the sharp turn of the trypsin-sensitive "hinge" region diminishes substrate binding and alters solubility. Zhao GP, et al. J Biol Chem, 1992 Jan 5. PMID 1309752

    See all (60) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. determined the crystal structures of apo-tryptophan synthase beta(2) and holo-beta(2) from Escherichia coli at 3.0 and 2.9 A resolutions, respectively.
      Title: Large conformational changes in the Escherichia coli tryptophan synthase beta(2) subunit upon pyridoxal 5'-phosphate binding.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to tryptophan synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    contributes_to tryptophan synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tryptophan synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tryptophan synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within aromatic amino acid family biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tryptophan biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tryptophan biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tryptophan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tryptophan biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tryptophan metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tryptophan synthase subunit beta
    NP_415777.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1317222..1318415 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415777.1 tryptophan synthase subunit beta [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415777.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      V9GZL4
      Conserved Domains (1) summary
      PRK04346
      Location:5 → 391
      PRK04346; tryptophan synthase subunit beta; Validated
    Nucleotide Protein
    Heading Accession and Version

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