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    cas2 CRISPR-associated endoribonuclease Cas2 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947213, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cas2
    Official Full Name
    CRISPR-associated endoribonuclease Cas2
    Primary source
    ECOCYC:EG12845
    Locus tag
    b2754
    See related
    ASAP:ABE-0009036
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2749; ygbF
    Summary
    YgbF(Cas2) belongs to the Pfam PF09707 family; six other PF09707 family members have been shown to be novel ssRNA endonucleases with ferrodoxin-like folds that cut U-rich regions (Beloglazova, 2008). [More information is available at EcoGene: EG12845]. Cas2 homologs in other organisms were shown to have sequence-specific endoribonuclease activity . [More information is available at EcoCyc: EG12845].
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    Genomic context

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    Sequence:
    NC_000913.3 (2878569..2878853, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene sulfate adenylyltransferase subunit 2 Neighboring gene alkaline phosphatase isozyme conversion protein Neighboring gene multifunctional nuclease Cas1 Neighboring gene pre-CRISPR RNA endonuclease Neighboring gene type I-E CRISPR system Cascade subunit CasD

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structures of the CRISPR genome integration complex. Wright AV, et al. Science, 2017 Sep 15. PMID 28729350, Free PMC Article
    2. Unusual nucleotide arrangement with repeated sequences in the Escherichia coli K-12 chromosome. Nakata A, et al. J Bacteriol, 1989 Jun. PMID 2656660, Free PMC Article
    3. Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK, et al. Nat Struct Mol Biol, 2014 Jun. PMID 24793649, Free PMC Article
    4. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Wang J, et al. Cell, 2015 Nov 5. PMID 26478180
    5. DNA binding specificities of Escherichia coli Cas1-Cas2 integrase drive its recruitment at the CRISPR locus. Moch C, et al. Nucleic Acids Res, 2017 Mar 17. PMID 28034956, Free PMC Article

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The presence of stx-phage was related to the molecular distribution of CRISPR in E. coli O26ratioH11 or NM. CRISPR might be a valuable biomarker to identify strains with high virulent potential.
      Title: [Association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia coli O26 ∶ H11 or NM].
    2. These results explain how the Cas1-Cas2 CRISPR integrase recognizes a sequence-dependent DNA structure to ensure site-selective CRISPR array expansion during the initial step of bacterial adaptive immunity.
      Title: Structures of the CRISPR genome integration complex.
    3. to form a long-lived ternary complex containing Cas1-Cas2, the acceptor DNA must carry a CRISPR locus, and the protospacer must not contain 3-single-stranded overhangs longer than 5 bases.
      Title: DNA binding specificities of Escherichia coli Cas1-Cas2 integrase drive its recruitment at the CRISPR locus.
    4. Asymmetric positioning of Cas1-Cas2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
      Title: Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
    5. These results establish Cas1-Cas2 as a trans-acting factor necessary for the recruitment and regulation of Cas3 at escape targets. Based on findings, a mechanistic framework is proposed describing how Cascade, Cas1, Cas2, and Cas3 work together to process and disable foreign genetic elements.
      Title: Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.
    6. To understand the molecular mechanisms of spacer acquisition, the crystal structure of E. coli Cas1-Cas2 bound with dual-forked DNA was determined; structure highlights the mechanistic principles related to new spacer acquisition, the protospacer DNA captured by Cas1-Cas2 adopts a dual-forked form, with the 3' overhangs of the protospacer essential for new spacer.
      Title: Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
    7. Cas1-Cas2 complexes specify sites of CRISPR spacer integration.
      Title: Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    General protein information

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    Preferred Names
    CRISPR-associated endoribonuclease Cas2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2878569..2878853 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417234.2 CRISPR-associated endoribonuclease Cas2 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417234.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3JDH7
      Conserved Domains (1) summary
      PRK11558
      Location:1 → 94
      PRK11558; putative ssRNA endonuclease; Provisional
    Nucleotide Protein
    Heading Accession and Version

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