usp ultraspiracle [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: uspprovided by FlyBase
Official Full Name: ultraspiracleprovided by FlyBase
Primary source: FLYBASE:FBgn0003964
Locus tag: Dmel_CG4380
See related: AllianceGenome:FB:FBgn0003964
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 2C; cf1; Cf1; CF1; CF1/Usp; CF1/USP; CG4380; Dmel\CG4380; dmUSP; DmUsp; DmUSP; dUSP; EG:22E5.1; l(1)2Cf; l(1)usp; NR2B4; Usp; USP; XR2C
Summary: Enables several functions, including core promoter sequence-specific DNA binding activity; nuclear receptor activity; and protein heterodimerization activity. Contributes to DNA-binding transcription activator activity, RNA polymerase II-specific and ecdysone binding activity. Involved in several processes, including border follicle cell migration; instar larval or pupal development; and nervous system development. Located in dendrite and nucleus. Part of activator ecdysone receptor complex. Is expressed in several structures, including adult cuticle; central nervous system; female germline cell; gland; and imaginal disc. Human ortholog(s) of this gene implicated in lung non-small cell carcinoma and pancreatic cancer. Orthologous to human RXRA (retinoid X receptor alpha); RXRB (retinoid X receptor beta); and RXRG (retinoid X receptor gamma). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 2C7-2C7; 1-0.5 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (2038798..2042311, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (1932831..1936344, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Context-dependent functional properties of EcR and USP receptors in Drosophila cell survival.
Title: Ligand-independent requirements of steroid receptors EcR and USP for cell survival.
nutrition controls ecdysone biosynthesis partially via FoxO-Usp prior to critical weight, ensuring that growth only stops once larvae have achieved a target nutritional status.
Title: Nutritional control of body size through FoxO-Ultraspiracle mediated ecdysone biosynthesis.
The results suggest that normal larval to pupal maturation in this mecopteran model insect requires the involvement of a distinct endocrine axis of USP binding to its own endogenous terpenoid ligand.
Title: Ligand binding pocket function of Drosophila USP is necessary for metamorphosis.
PKC mediates USP phosphorylation at Ser35 in Kc and S2 cells, which regulates 20-hydroxyecdysone signaling.
Title: PKC-mediated USP phosphorylation at Ser35 modulates 20-hydroxyecdysone signaling in Drosophila.
sumoylation of Usp protein can be an important factor in modulating its activity by changing molecular interactions
Title: Alternative sumoylation sites in the Drosophila nuclear receptor Usp.
Ecdysone and NO mediate gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development
Title: Ecdysone- and NO-mediated gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development.
Ecdysone receptor and USP were expressed in numerous adult tissues, but receptor activity varied depending on tissue type and adult age. Receptor activity is reduced when a constitutively inactive ecdysone receptor is present.
Title: Ecdysone receptor expression and activity in adult Drosophila melanogaster.
Data show that ultraspiracle- and ecdysteroid receptor-mediated transcriptional activity not only depends on an interaction between the ecdysteroid receptor and a given hormone response element, but is also modified by surrounding DNA sequences.
Title: The N-terminus of ecdysteroid receptor isoforms and ultraspiracle interacts with different ecdysteroid response elements in a sequence specific manner to modulate transcriptional activity.
stability of the ecdysone receptor (EcR) is regulated in a cooperative manner by the AB-domains of EcR and Ultraspiracle
Title: Transcriptional activity of ecdysone receptor isoforms is regulated by modulation of receptor stability and interaction with Ab- and C-domains of the heterodimerization partner ultraspiracle.
The heterodimerization partner Usp increased DNA binding of ecdysone receptor considerably
Title: DNA-binding properties of Drosophila ecdysone receptor isoforms and their modification by the heterodimerization partner ultraspiracle.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1871 (e-PCR)
- UniSTS:70290

D1S3696 (e-PCR)
- UniSTS:474274

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to DNA-binding transcription activator activity, RNA polymerase II-specific	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables core promoter sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to ecdysone binding	IDA Inferred from Direct Assay more info	PubMed
enables hormone binding	IDA Inferred from Direct Assay more info	PubMed
enables lipid binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclear receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear receptor activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to nuclear receptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables nuclear receptor activity	NAS Non-traceable Author Statement more info	PubMed
enables nuclear steroid receptor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein heterodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to ecdysone	IDA Inferred from Direct Assay more info	PubMed
involved_in dendrite morphogenesis	NAS Non-traceable Author Statement more info	PubMed
involved_in ecdysone biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ecdysone receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metamorphosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mushroom body development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of cell differentiation	TAS Traceable Author Statement more info	PubMed
involved_in nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in pupariation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of development, heterochronic	TAS Traceable Author Statement more info	PubMed
involved_in regulation of organ growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to ecdysone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinoic acid receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of activator ecdysone receptor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of activator ecdysone receptor complex	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IMP Inferred from Mutant Phenotype more info	PubMed
part_of ecdysone receptor holocomplex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of ecdysone receptor holocomplex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in polytene chromosome	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: ultraspiracle

Names: CG4380-PA; CG4380-PB; X receptor at 2C; chorion factor 1; chorion factor CF1; ultraspiricle; usp-PA; usp-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

2038798..2042311 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001272239.2 → NP_001259168.1 ultraspiracle, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259168.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P20153, Q9W535

UniProtKB/TrEMBL

M9PGS2

Conserved Domains (2) summary

cd06943
Location:237 → 480

NR_LBD_RXR_like; The ligand binding domain of the retinoid X receptor and Ultraspiracle, members of nuclear receptor superfamily

cd06956
Location:102 → 178

NR_DBD_RXR; DNA-binding domain of retinoid X receptor (RXR) is composed of two C4-type zinc fingers
NM_057433.4 → NP_476781.1 ultraspiracle, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476781.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P20153, Q9W535

UniProtKB/TrEMBL

M9PGS2

Related

FBpp0070332

Conserved Domains (2) summary

cd06943
Location:237 → 480

NR_LBD_RXR_like; The ligand binding domain of the retinoid X receptor and Ultraspiracle, members of nuclear receptor superfamily

cd06956
Location:102 → 178

NR_DBD_RXR; DNA-binding domain of retinoid X receptor (RXR) is composed of two C4-type zinc fingers