Slbp Stem-loop binding protein [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Slbpprovided by FlyBase
Official Full Name: Stem-loop binding proteinprovided by FlyBase
Primary source: FLYBASE:FBgn0041186
Locus tag: Dmel_CG11886
See related: AllianceGenome:FB:FBgn0041186
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG11886; Dmel\CG11886; dSLBP; dSLPB; slbp; SLBP
Summary: Enables RNA stem-loop binding activity and sequence-specific mRNA binding activity. Involved in mRNA 3'-end processing by stem-loop binding activity and cleavage and mitotic chromosome condensation. Located in cytoplasm and nucleus. Is expressed in several structures, including CNS glial cell; ganglia; head epidermis primordium; mesoderm; and presumptive embryonic/larval system. Orthologous to human SLBP (stem-loop histone mRNA binding protein). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 98F13-98F13; 3-97 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (29152074..29153266, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (24977796..24978988, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Although the C-terminal tail of dSLBP does not contact the RNA, phosphorylation of the tail promotes SLBP conformations competent for RNA binding and thereby appears to reduce the entropic penalty for the association.
Title: Molecular mechanisms for the regulation of histone mRNA stem-loop-binding protein by phosphorylation.
nuclear retention involves Chk2-mediated inactivation of the Drosophila stem loop binding protein (SLBP), the levels of which are specifically depleted in damaged nuclei following Chk2 phosphorylation, an event that contributes to nuclear fallout.
Title: Developmentally regulated elimination of damaged nuclei involves a Chk2-dependent mechanism of mRNA nuclear retention.
The nuclear magnetic resonance and kinetic studies presented here provide a framework for understanding how SLBP recognizes histone mRNA and highlight possible structural roles of phosphorylation and proline isomerization in RNA binding proteins
Title: Interaction of the histone mRNA hairpin with stem-loop binding protein (SLBP) and regulation of the SLBP-RNA complex by phosphorylation and proline isomerization.
Slbp mutants display genomic instability, including loss of heterozygosity (LOH), increased presence of chromosome breaks, tetraploidy, and changes in position effect variegation (PEV).
Title: Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation.
Removal of the phosphoryl group from T230 by either dephosphorylation or mutation results in a 7-fold reduction in the affinity of SLBP for the stem-loop RNA
Title: Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding.
Phosphorylation of T120 and T230 site of Stem-loop binding protein (SLBP) differentially affects the ability of the protein to restore viability and histone mRNA processing.
Title: Drosophila stem-loop binding protein intracellular localization is mediated by phosphorylation and is required for cell cycle-regulated histone mRNA expression.
electrostatic effect of protein phosphorylation can be partially mimicked by a mutant form of SLBP wherein four C-terminal serines are replaced with glutamic acids
Title: Electrostatic contribution of serine phosphorylation to the Drosophila SLBP--histone mRNA complex.
characterize the structural and dynamic features of the N-terminal domain of SLBP from Drosophila
Title: The N-terminal domain of the Drosophila histone mRNA binding protein, SLBP, is intrinsically disordered with nascent helical structure.
Developmental control of histone mRNA and dSLBP synthesis during Drosophila embryogenesis and the role of dSLBP in histone mRNA 3' end processing in vivo.
Title: Developmental control of histone mRNA and dSLBP synthesis during Drosophila embryogenesis and the role of dSLBP in histone mRNA 3' end processing in vivo.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA stem-loop binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA stem-loop binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone pre-mRNA stem-loop binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific mRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in mRNA 3'-end processing by stem-loop binding and cleavage	IBA Inferred from Biological aspect of Ancestor more info
involved_in mRNA 3'-end processing by stem-loop binding and cleavage	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA 3'-end processing by stem-loop binding and cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
part_of histone pre-mRNA 3'end processing complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Stem-loop binding protein

Names: CG11886-PA; Slbp-PA; stem loop binding protein; stem-loop-binding protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

29152074..29153266 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_058132.3 → NP_477480.1 Stem-loop binding protein [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477480.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9GU71, Q9VAN6

Related

FBpp0084778

Conserved Domains (1) summary

pfam15247
Location:191 → 256

SLBP_RNA_bind; Histone RNA hairpin-binding protein RNA-binding domain