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    CHAF1A chromatin assembly factor 1 subunit A [ Homo sapiens (human) ]

    Gene ID: 10036, updated on 10-Dec-2024

    Summary

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    Official Symbol
    CHAF1Aprovided by HGNC
    Official Full Name
    chromatin assembly factor 1 subunit Aprovided by HGNC
    Primary source
    HGNC:HGNC:1910
    See related
    Ensembl:ENSG00000167670 MIM:601246; AllianceGenome:HGNC:1910
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CAF1; P150; CAF-1; CAF1B; CAF1P150
    Summary
    Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]
    Expression
    Broad expression in bone marrow (RPKM 7.4), testis (RPKM 6.1) and 24 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See CHAF1A in Genome Data Viewer
    Location:
    19p13.3
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (4402640..4450830)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (4386080..4434290)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (4402637..4443394)

    Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4350305-4350806 Neighboring gene eukaryotic translation initiation factor 1 pseudogene 6 Neighboring gene MPN domain containing Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4365919-4366840 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4368989-4369938 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4369939-4370886 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4372431-4373164 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4373165-4373898 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancers GRCh37_chr19:4374633-4375365 and GRCh37_chr19:4375366-4376099 Neighboring gene SH3 domain containing GRB2 like 1, endophilin A2 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4377567-4378299 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13771 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13772 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13773 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:4386482-4386982 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4389401-4390008 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4390009-4390615 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4390616-4391224 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4394991-4395501 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4399905-4400734 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4402397-4403094 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4403095-4403790 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4408505-4409316 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4409317-4410128 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4412803-4413801 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:4430727-4431490 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4436676-4437176 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4437177-4437677 Neighboring gene Sharpr-MPRA regulatory region 9621 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:4444373-4444541 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4453327-4454026 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9890 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13774 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9891 Neighboring gene microRNA 4746 Neighboring gene UBX domain protein 6 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13775 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13776 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9892 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9893 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4474209-4475158 Neighboring gene HDGF like 2 Neighboring gene perilipin 4 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4534387-4534942 Neighboring gene perilipin 5

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Unorthodox PCNA Binding by Chromatin Assembly Factor 1. Gopinathan Nair A, et al. Int J Mol Sci, 2022 Sep 21. PMID 36232396, Free PMC Article
    2. Emerging roles of the histone chaperone CAF-1 in cellular plasticity. Cheloufi S, et al. Curr Opin Genet Dev, 2017 Oct. PMID 28692904, Free PMC Article
    3. Over-expression of CHAF1A in Epithelial Ovarian Cancer can promote cell proliferation and inhibit cell apoptosis. Xia D, et al. Biochem Biophys Res Commun, 2017 Apr 22. PMID 28286267
    4. The Chromatin Assembly Factor Complex 1 (CAF1) and 5-Azacytidine (5-AzaC) Affect Cell Motility in Src-transformed Human Epithelial Cells. Endo A, et al. J Biol Chem, 2017 Jan 6. PMID 27872192, Free PMC Article
    5. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Yang C, et al. Nucleic Acids Res, 2017 Jan 25. PMID 27794043, Free PMC Article

    See all (149) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Inhibition of CAF-1 histone chaperone complex triggers cytosolic DNA and dsRNA sensing pathways and induces intrinsic immunity of hepatocellular carcinoma.
      Title: Inhibition of CAF-1 histone chaperone complex triggers cytosolic DNA and dsRNA sensing pathways and induces intrinsic immunity of hepatocellular carcinoma.
    2. Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
      Title: Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
    3. Aberrant SPOP-CHAF1A ubiquitination axis triggers tumor autophagy that endows a therapeutical vulnerability in diffuse large B cell lymphoma.
      Title: Aberrant SPOP-CHAF1A ubiquitination axis triggers tumor autophagy that endows a therapeutical vulnerability in diffuse large B cell lymphoma.
    4. CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.
      Title: CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.
    5. CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
      Title: CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
    6. Detection of CAF-1/p60 in peripheral blood as a potential biomarker of HNSCC tumors.
      Title: Detection of CAF-1/p60 in peripheral blood as a potential biomarker of HNSCC tumors.
    7. DNA methylation mediated downregulation of histone H3 variant H3.3 affects cell proliferation contributing to the development of HCC.
      Title: DNA methylation mediated downregulation of histone H3 variant H3.3 affects cell proliferation contributing to the development of HCC.
    8. Identification and external validation of a prognostic signature associated with DNA repair genes in gastric cancer.
      Title: Identification and external validation of a prognostic signature associated with DNA repair genes in gastric cancer.
    9. CHAF1A overexpression in human retinoblastoma promotes cell proliferation and suppresses apoptosis.
      Title: CHAF1A overexpression in human retinoblastoma promotes cell proliferation and suppresses apoptosis.
    10. Histone Loaders CAF1 and HIRA Restrict Epstein-Barr Virus B-Cell Lytic Reactivation.
      Title: Histone Loaders CAF1 and HIRA Restrict Epstein-Barr Virus B-Cell Lytic Reactivation.
    Submit: New GeneRIF Correction See all GeneRIFs (36)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of chromatin assembly factor 1, subunit A (CHAF1A) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120 is identified to have a physical interaction with chromatin assembly factor 1, subunit A (CHAF1A; CAF1B) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
    Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 is identified to have a physical interaction with chromatin assembly factor 1, subunit A (CHAF1A; CAF1B) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC71229

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables chromatin binding TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables chromo shadow domain binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables unfolded protein binding TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication-dependent chromatin assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in nucleosome assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleosome assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of CAF-1 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of CAF-1 complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of CAF-1 complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    chromatin assembly factor 1 subunit A
    Names
    CAF-1 subunit A
    CAF-I 150 kDa subunit
    CAF-I p150
    CTB-50L17.7
    chromatin assembly factor I (150 kDa)
    chromatin assembly factor I p150 subunit
    hp150

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_005483.3 → NP_005474.2  chromatin assembly factor 1 subunit A

      See identical proteins and their annotated locations for NP_005474.2

      Status: VALIDATED

      Source sequence(s)
      AA576757, AC011498, BC052620, BE796132, BG775588, BM826511, BQ889365, H98924, U20979
      Consensus CDS
      CCDS32875.1
      UniProtKB/Swiss-Prot
      Q13111, Q6NXG5, Q7Z7K3, Q9UJY8
      Related
      ENSP00000301280.4, ENST00000301280.10
      Conserved Domains (4) summary
      pfam11600
      Location:320 → 479
      CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal
      pfam12253
      Location:558 → 601
      CAF1A; Chromatin assembly factor 1 subunit A
      pfam15539
      Location:665 → 954
      CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
      pfam15557
      Location:1 → 226
      CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

      Range
      4402640..4450830
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011527605.3 → XP_011525907.1  chromatin assembly factor 1 subunit A isoform X1

      Conserved Domains (4) summary
      pfam11600
      Location:320 → 479
      CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal
      pfam12253
      Location:558 → 601
      CAF1A; Chromatin assembly factor 1 subunit A
      pfam15539
      Location:665 → 943
      CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
      pfam15557
      Location:1 → 226
      CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA

    2. XM_011527607.3 → XP_011525909.1  chromatin assembly factor 1 subunit A isoform X2

      Conserved Domains (4) summary
      pfam11600
      Location:382 → 460
      CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal
      pfam12253
      Location:539 → 582
      CAF1A; Chromatin assembly factor 1 subunit A
      pfam15539
      Location:646 → 935
      CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
      pfam15557
      Location:1 → 226
      CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA

    3. XM_047438011.1 → XP_047293967.1  chromatin assembly factor 1 subunit A isoform X3

    4. XM_047438012.1 → XP_047293968.1  chromatin assembly factor 1 subunit A isoform X3

    5. XM_047438013.1 → XP_047293969.1  chromatin assembly factor 1 subunit A isoform X3

    RNA

    1. XR_007066505.1 RNA Sequence

    2. XR_936135.3 RNA Sequence

    3. XR_007066507.1 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060943.1 Alternate T2T-CHM13v2.0

      Range
      4386080..4434290
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054319464.1 → XP_054175439.1  chromatin assembly factor 1 subunit A isoform X1

    2. XM_054319465.1 → XP_054175440.1  chromatin assembly factor 1 subunit A isoform X4

    3. XM_054319467.1 → XP_054175442.1  chromatin assembly factor 1 subunit A isoform X5

    4. XM_054319466.1 → XP_054175441.1  chromatin assembly factor 1 subunit A isoform X2

    5. XM_054319468.1 → XP_054175443.1  chromatin assembly factor 1 subunit A isoform X3

    6. XM_054319469.1 → XP_054175444.1  chromatin assembly factor 1 subunit A isoform X3

    RNA

    1. XR_008485038.1 RNA Sequence

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q13111.3 GenPept UniProtKB/Swiss-Prot:Q13111

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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