ROT1 Rot1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ROT1
Official Full Name: Rot1p
Primary source: SGD:S000004813
Locus tag: YMR200W
See related: AllianceGenome:SGD:S000004813; FungiDB:YMR200W; VEuPathDB:YMR200W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables unfolded protein binding activity. Involved in several processes, including budding cell apical bud growth; protein folding; and protein glycosylation. Located in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ROT1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (664752..665522)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rot1 needs the transmembrane Ser250 to interact with other membrane components and to exert its functional role.
Title: A transmembrane serine residue in the Rot1 protein is essential for yeast cell viability.
the role of Rot1 as a chaperone required to ensure proper glycosylation is demonstrated in yeast
Title: The essential endoplasmic reticulum chaperone Rot1 is required for protein N- and O-glycosylation in yeast.
The results show that two regions, amino acids 26-60 and 200-228, are involved in the post-translational translocation of Rot1 across the endoplasmic reticulum membrane.
Title: Targeting and membrane insertion into the endoplasmic reticulum membrane of Saccharomyces cerevisiae essential protein Rot1.
is an essential molecular chaperone in the endoplasmic reticulum
Title: Saccharomyces cerevisiae Rot1 is an essential molecular chaperone in the endoplasmic reticulum.
Characterization of the post-translational modification, transport and membrane topology of Rot1 is reported.
Title: Membrane topology and post-translational modification of the Saccharomyces cerevisiae essential protein Rot1.
study revealed a role for Rot1 in cell morphogenesis during cell cycle progression; functional antagonism between Rot1 and Clb2 is supported by genetic and biochemical data, which indicate that Rot1 is implicated in Clb2 protein degradation
Title: Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycle.
Rot1p participates in protein folding with Kar2p.
Title: Saccharomyces cerevisiae Rot1p is an ER-localized membrane protein that may function with BiP/Kar2p in protein folding.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in 'de novo' protein folding	IBA Inferred from Biological aspect of Ancestor more info
involved_in 'de novo' protein folding	IEA Inferred from Electronic Annotation more info
involved_in 'de novo' protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in 'de novo' protein folding	IPI Inferred from Physical Interaction more info	PubMed
involved_in budding cell apical bud growth	IBA Inferred from Biological aspect of Ancestor more info
involved_in budding cell apical bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in budding cell apical bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of actin cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein N-linked glycosylation	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein O-linked mannosylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein folding	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein folding in endoplasmic reticulum	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rot1p

NP_013927.1

Molecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface