Hcls1 hematopoietic cell specific Lyn substrate 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hcls1provided by MGI
Official Full Name: hematopoietic cell specific Lyn substrate 1provided by MGI
Primary source: MGI:MGI:104568
See related: Ensembl:ENSMUSG00000022831 AllianceGenome:MGI:104568
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: HS1
Summary: Enables SH3 domain binding activity. Involved in several processes, including erythrocyte differentiation; positive regulation of myeloid leukocyte differentiation; and regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Is expressed in thymus primordium. Orthologous to human HCLS1 (hematopoietic cell-specific Lyn substrate 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in spleen adult (RPKM 53.6), thymus adult (RPKM 37.7) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 B3; 16 26.3 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (36755345..36783576)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (36934983..36963214)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HS1 and Arp2/3 complex-mediated actin polymerization are essential for the most efficient dendritic cell random migration and force generation.
Title: The Arp2/3 complex binding protein HS1 is required for efficient dendritic cell random migration and force generation.
HS1 as an important regulator of proper Rac1 and Rap1 activation and neutrophil extravasation.
Title: HS1 deficiency impairs neutrophil recruitment in vivo and activation of the small GTPases Rac1 and Rap1.
Results identified two proteins P23 and HCLS1, which were not known as RNA-binding proteins, exhibiting RNA-binding activity.
Title: Identification of RNA-binding Proteins in Macrophages by Interactome Capture.
Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.
Title: Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.
studies show that HS1 promotes an early step in the endocytic pathway that is required for efficient Ag presentation of exogenous Ag by dendritic cells
Title: The actin regulatory protein HS1 is required for antigen uptake and presentation by dendritic cells.
Studies show that HS1 functions in concert with WASp to fine-tune DC cytoarchitecture and direct cell migration.
Title: Hematopoietic lineage cell-specific protein 1 functions in concert with the Wiskott-Aldrich syndrome protein to promote podosome array organization and chemotaxis in dendritic cells.
In vivo, immunodeficient Rag2(-/-)gamma(c)(-/-) mice injected with HS1-silenced CLL B cells showed a decreased organ infiltration with the notable exception of the bone marrow.
Title: HS1 has a central role in the trafficking and homing of leukemic B cells.
Hematopoietic lineage cell specific protein 1 plays a significant functional role in platelet activation downstream of glycoprotein VI and protease-activated receptors[Hematopoietic lineage cell specific protein 1]
Title: Hematopoietic lineage cell specific protein 1 (HS1) is a functionally important signaling molecule in platelet activation.
HS1 functions as an effector of Itk in the T cell actin-regulatory pathway, and directs the spatial organization of PLCgamma1 signaling complexes
Title: Hematopoietic lineage cell-specific protein 1 is recruited to the immunological synapse by IL-2-inducible T cell kinase and regulates phospholipase Cgamma1 Microcluster dynamics during T cell spreading.
tyrosine phosphorylation of HS1 occurs downstream of both PAR-1 and PAR-4. HS1 phosphorylation is a Gq mediated response regulated by Src kinases
Title: Hematopoietic lineage cell-specific protein-1 (HS1) regulates PAR-mediated ERK activation and thromboxane generation in platelets.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB203030 (e-PCR)
- UniSTS:209216

AW213261 (e-PCR)
- UniSTS:180906

X84797 (e-PCR)
- UniSTS:163572

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables SH3 domain binding	IDA Inferred from Direct Assay more info	PubMed
enables SH3 domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling adaptor activity	IEA Inferred from Electronic Annotation more info
enables signaling adaptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in actin filament organization	IEA Inferred from Electronic Annotation more info
involved_in actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to cytokine stimulus	ISO Inferred from Sequence Orthology more info
involved_in erythrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in granulocyte colony-stimulating factor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in granulocyte colony-stimulating factor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of leukocyte apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of leukocyte apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear transport	IEA Inferred from Electronic Annotation more info
involved_in nuclear transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of granulocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of granulocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of peptidyl-tyrosine phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein import into nucleus	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin filament polymerization	IEA Inferred from Electronic Annotation more info
involved_in regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in response to hormone	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with actin filament	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cortical actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in site of polarized growth	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: hematopoietic lineage cell-specific protein

Names: hematopoietic cell-specific LYN substrate 1; lckBP1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008225.2 → NP_032251.2 hematopoietic lineage cell-specific protein

Status: VALIDATED

Source sequence(s)

AC154763, AK040049, BX519502, CA321698

Consensus CDS

CCDS37336.1

UniProtKB/Swiss-Prot

P49710, Q922I8

UniProtKB/TrEMBL

Q542G0

Related

ENSMUSP00000023531.9, ENSMUST00000023531.15

Conserved Domains (2) summary

pfam02218
Location:119 → 154

HS1_rep; Repeat in HS1/Cortactin

cl17036
Location:437 → 485

SH3; Src Homology 3 domain superfamily