PNP1 purine-nucleoside phosphorylase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PNP1
Official Full Name: purine-nucleoside phosphorylase
Primary source: SGD:S000004199
Locus tag: YLR209C
See related: AllianceGenome:SGD:S000004199; FungiDB:YLR209C; VEuPathDB:YLR209C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables inosine nucleosidase activity; nicotinamide riboside hydrolase activity; and purine-nucleoside phosphorylase activity. Involved in purine ribonucleoside catabolic process and pyridine nucleotide biosynthetic process. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in purine nucleoside phosphorylase deficiency and purine-pyrimidine metabolic disorder. Orthologous to human PNP (purine nucleoside phosphorylase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PNP1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (560797..561732, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The mechanism of action of nicotinamide riboside is totally dependent on increased net NAD synthesis through two pathways, the Nrk1 pathway and the Urh1/Pnp1/Meu1 pathway, which is Nrk1 independent.
Title: Nicotinamide riboside promotes Sir2 silencing and extends lifespan via Nrk and Urh1/Pnp1/Meu1 pathways to NAD+.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables inosine nucleosidase activity	IDA Inferred from Direct Assay more info	PubMed
enables nicotinamide riboside hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables purine-nucleoside phosphorylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables purine-nucleoside phosphorylase activity	IDA Inferred from Direct Assay more info	PubMed
enables purine-nucleoside phosphorylase activity	IEA Inferred from Electronic Annotation more info
enables purine-nucleoside phosphorylase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in NAD biosynthesis via nicotinamide riboside salvage pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in guanosine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inosine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nicotinate nucleotide salvage	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleoside metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IC Inferred by Curator more info	PubMed

General protein information

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Preferred Names: purine-nucleoside phosphorylase

NP_013310.1

EC 2.4.2.1

Purine nucleoside phosphorylase; specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

560797..561732 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182096.1 → NP_013310.1 TPA: purine-nucleoside phosphorylase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013310.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYL0, Q05788

UniProtKB/TrEMBL

A7A197, B3RH98, B5VNC7, C7GIT8, C8ZDH3, G2WJ39, N1P1C1

Conserved Domains (1) summary

cd09009
Location:12 → 307

PNP-EcPNPII_like; purine nucleoside phosphorylases similar to human PNP and Escherichia coli PNP-II (XapA)