TPK2 cAMP-dependent protein kinase catalytic subunit TPK2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TPK2
Official Full Name: cAMP-dependent protein kinase catalytic subunit TPK2
Primary source: SGD:S000006124
Locus tag: YPL203W
See related: AllianceGenome:SGD:S000006124; FungiDB:YPL203W; VEuPathDB:YPL203W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PKA2; PKA3; YKR1
Summary: Enables cAMP-dependent protein kinase activity. Involved in several processes, including intracellular signal transduction; invasive growth in response to glucose limitation; and negative regulation of cytoplasmic mRNA processing body assembly. Located in several cellular components, including P-body; chromatin; and cytosol. Part of cAMP-dependent protein kinase complex. Human ortholog(s) of this gene implicated in adrenal cortical adenoma; congestive heart failure; fibrolamellar carcinoma; platelet-type bleeding disorder 19; and primary pigmented nodular adrenocortical disease 4. Orthologous to several human genes including PRKACA (protein kinase cAMP-activated catalytic subunit alpha); PRKACB (protein kinase cAMP-activated catalytic subunit beta); and PRKACG (protein kinase cAMP-activated catalytic subunit gamma). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TPK2 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (166256..167398)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression.
Title: Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression.
A prion-like domain of Tpk2 catalytic subunit of protein kinase A modulates P-body formation in response to stress in budding yeast.
Title: A prion-like domain of Tpk2 catalytic subunit of protein kinase A modulates P-body formation in response to stress in budding yeast.
active participation of SSA1 in the degradation of misfolded proteins establishes an essential role of Hsp70 in deciding client fate during stress.
Title: Hsp70 clears misfolded kinases that partitioned into distinct quality-control compartments.
Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression.
Title: The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae.
Tpk2 and Tpk3 are associated with translation initiation factors Pab1 and Rps3 in exponentially growing cells.
Title: PKA isoforms coordinate mRNA fate during nutrient starvation.
Trehalase activation is associated with phosphorylation of typical PKA sites.
Title: In vivo phosphorylation of Ser21 and Ser83 during nutrient-induced activation of the yeast protein kinase A (PKA) target trehalase.
Tpk2 and Tpk1 interact with Pkh1 through a hydrophobic motif-PDK1-interacting fragment (PIF) pocket interaction.
Title: The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae.
TPK2 regulates spore formation via Ras/cyclic AMP/protein kinase A pathway.
Title: Acetate regulation of spore formation is under the control of the Ras/cyclic AMP/protein kinase A pathway and carbon dioxide in Saccharomyces cerevisiae.
Data show that the Ras2p localization is regulated by PKA, and that PKA down-regulates Ras2p activity and the protein association between Cdc25p and Ras2-GTP, which is due to a reduced Ras2-GEF Cdc25p activity.
Title: The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae.
phosphatidylserine synthase is phosphorylated on Ser(46) and Ser(47) by protein kinase A, which results in a higher amount of enzyme for the net effect of stimulating the synthesis of phosphatidylserine
Title: Phosphorylation of yeast phosphatidylserine synthase by protein kinase A: identification of Ser46 and Ser47 as major sites of phosphorylation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G1 to G0 transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Ras protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in invasive growth in response to glucose limitation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cytoplasmic mRNA processing body assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cytoplasmic translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein kinase A signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of cAMP-dependent protein kinase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cAMP-dependent protein kinase catalytic subunit TPK2

NP_015121.1

EC 2.7.11.11

cAMP-dependent protein kinase catalytic subunit; promotes nutrient responsive growth via the Ras-cAMP signaling pathway; phosphorylates and inhibits histone demethylase Jhd2p in response to glucose, promoting H3K4 trimethylation and maintaining H3K14 acetylation by preventing chromatin binding of Rpd3p; required for Sfl1p-mediated pseudohyphal growth; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

166256..167398

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184017.1 → NP_015121.1 TPA: cAMP-dependent protein kinase catalytic subunit TPK2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015121.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3G6, P06245

UniProtKB/TrEMBL

A6ZW51, B3LKM6, B5VSX7, C7GN31, N1NVN4

Conserved Domains (1) summary

cd05580
Location:69 → 358

STKc_PKA_like; Catalytic subunit of the Serine/Threonine Kinases, cAMP-dependent protein kinases