STE50 Ste50p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STE50
Official Full Name: Ste50p
Primary source: SGD:S000000537
Locus tag: YCL032W
See related: AllianceGenome:SGD:S000000537; FungiDB:YCL032W; VEuPathDB:YCL032W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables SAM domain binding activity and protein kinase regulator activity. Involved in intracellular signal transduction; pheromone-dependent signal transduction involved in conjugation with cellular fusion; and signal transduction involved in filamentous growth. Acts upstream of or within establishment of cell polarity. Located in cytoplasm. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See STE50 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (63441..64481)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network.
Title: Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor.
Two peptide motifs within the intrinsically disordered cytoplasmic tail of Opy2p physically interact with the Step50p-RA domain.
Title: Binding the atypical RA domain of Ste50p to the unfolded Opy2p cytoplasmic tail is essential for the high-osmolarity glycerol pathway.
Ste50 has a role in control of MAPK specificity by feedback phosphorylation
Title: Control of MAPK specificity by feedback phosphorylation of shared adaptor protein Ste50.
inactivation of STE50 influences stress tolerance in mutants of the Ras-cAMP pathway, which is a major determinant of intrinsic stress tolerance and cell survival
Title: Ste50 adaptor protein influences Ras/cAMP-driven stress-response and cell survival in Saccharomyces cerevisiae.
propose that Opy2p can serve as a membrane anchor for the Ste50p/Ste11p module in the activation of the HOG pathway
Title: Adaptor protein Ste50p links the Ste11p MEKK to the HOG pathway through plasma membrane association.
Yeast bearing mutations that prevented the heterotypic Ste11-Ste50 association in vitro presented signaling defects in the mating and high-osmolarity growth pathways
Title: Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction.
Results describe the solution structure of the sterile alpha motifs (SAM) domain of Ste11 and its interactions with the cognate SAM domain-containing region of Ste50.
Title: Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex.
Ste50 serves as an adaptor to tether Ste11 to the plasma membrane.
Title: The RA domain of Ste50 adaptor protein is required for delivery of Ste11 to the plasma membrane in the filamentous growth signaling pathway of the yeast Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables SAM domain binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase regulator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within establishment of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in osmosensory signaling pathway via Sho1 osmosensor	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in p38MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in p38MAPK cascade	IPI Inferred from Physical Interaction more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IEA Inferred from Electronic Annotation more info
involved_in response to pheromone	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in signal transduction involved in filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Ste50p

NP_009898.1

Adaptor protein for various signaling pathways; involved in the mating response, invasive/filamentous growth and osmotolerance; acts as an adaptor that links the G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction; localizes to cortical patches at the site of shmoo initiation, facilitating polarity establishment, and remaining at the shmoo tip during mating

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

63441..64481

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178677.1 → NP_009898.1 TPA: Ste50p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009898.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQY3, P25344, Q3S1N2

UniProtKB/TrEMBL

A6ZTF8, B3LU50, B5VES6, C7GSY9, G2W9Z3, N1P660

Conserved Domains (2) summary

cd01786
Location:226 → 325

RA_STE50; Ras-associating (RA) domain found in the fungal adaptor protein STE50

pfam09235
Location:30 → 104

Ste50p-SAM; Ste50p, sterile alpha motif