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    phoQ sensor histidine kinase PhoQ [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946326, updated on 3-Dec-2024

    Summary

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    Official Symbol
    phoQ
    Official Full Name
    sensor histidine kinase PhoQ
    Primary source
    ECOCYC:EG10732
    Locus tag
    b1129
    See related
    ASAP:ABE-0003805
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1115
    Summary
    PhoPQ has been primarily characterized in Salmonella, with significant differences from Escherichia coli. [More information is available at EcoGene: EG10732]. The subunit structure in vivo is not known. [More information is available at EcoCyc: EG10732].
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    Genomic context

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    Sequence:
    NC_000913.3 (1188316..1189776, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene peptidase T Neighboring gene 50S ribosomal protein L16-arginine 3-hydroxylase Neighboring gene DNA-binding transcriptional dual regulator PhoP Neighboring gene adenylosuccinate lyase Neighboring gene lysogenization regulator

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Atomic insights into the signaling landscape of E. coli PhoQ histidine kinase from molecular dynamics simulations. Lazaridi S, et al. Sci Rep, 2024 Jul 26. PMID 39085378, Free PMC Article
    2. The small membrane protein MgrB regulates PhoQ bifunctionality to control PhoP target gene expression dynamics. Salazar ME, et al. Mol Microbiol, 2016 Nov. PMID 27447896
    3. Cys-scanning disulfide crosslinking and bayesian modeling probe the transmembrane signaling mechanism of the histidine kinase, PhoQ. Molnar KS, et al. Structure, 2014 Sep 2. PMID 25087511, Free PMC Article
    4. Crystal structure of a functional dimer of the PhoQ sensor domain. Cheung J, et al. J Biol Chem, 2008 May 16. PMID 18348979, Free PMC Article
    5. Determination of the physiological dimer interface of the PhoQ sensor domain. Goldberg SD, et al. J Mol Biol, 2008 Jun 13. PMID 18468622, Free PMC Article

    See all (49) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Atomic insights into the signaling landscape of E. coli PhoQ histidine kinase from molecular dynamics simulations.
      Title: Atomic insights into the signaling landscape of E. coli PhoQ histidine kinase from molecular dynamics simulations.
    2. data suggest that MgrB affects the acid resistance of E. coli by modulating the expression of iraM, but not completely through PhoP/Q.
      Title: MgrB affects the acid stress response of Escherichia coli by modulating the expression of iraM.
    3. The small inner membrane protein SafA activates PhoQ in Escherichia coli independently from other known signals that control PhoQ activity.
      Title: Identification of an internal cavity in the PhoQ sensor domain for PhoQ activity and SafA-mediated control.
    4. findings directly link the PhoQ/PhoP two-component system to bacterial osmosensing
      Title: Osmosensing by the bacterial PhoQ/PhoP two-component system.
    5. Both MicA and GcvB bind phoPQ mRNA in vivo and in vitro around the translation initiation region of phoP. Binding of either small RNA is sufficient to inhibit ribosome binding and induce mRNA degradation
      Title: Post-transcriptional control of the Escherichia coli PhoQ-PhoP two-component system by multiple sRNAs involves a novel pairing region of GcvB.
    6. Site-directed mutagenesis studies revealed that R53, within SafA(41-65), was important for the activation of PhoQ, and D179 of the PhoQ sensor domain was required for its activation by SafA.
      Title: The connector SafA interacts with the multi-sensing domain of PhoQ in Escherichia coli.
    7. Taken together, these results demonstrate that a decrease in the oxidizing activity of the periplasm stimulates PhoQ/PhoP and may reveal a new input stimulus for this important two-component system.
      Title: Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli.
    8. MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competing with ribosome binding.
      Title: MicA sRNA links the PhoP regulon to cell envelope stress.
    9. analysis indicated that PhoQ kinase function is dependent on the polarity of Asn202, rather than its precise structure or position in the transmembrane region.
      Title: Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ.
    10. Expression of MgrR requires the PhoQ/PhoP two-component system.
      Title: A PhoQ/P-regulated small RNA regulates sensitivity of Escherichia coli to antimicrobial peptides.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phosphoprotein phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphoprotein phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphorelay sensor kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphorelay sensor kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within cellular response to magnesium starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within osmosensory signaling via phosphorelay pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within signal transduction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sensor histidine kinase PhoQ
    NP_415647.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1188316..1189776 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415647.1 sensor histidine kinase PhoQ [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415647.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2W6P6Y6
      Conserved Domains (1) summary
      PRK10815
      Location:3 → 485
      PRK10815; two-component system sensor histidine kinase PhoQ
    Nucleotide Protein
    Heading Accession and Version

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