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    murD UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944818, updated on 3-Dec-2024

    Summary

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    Official Symbol
    murD
    Official Full Name
    UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase
    Primary source
    ECOCYC:EG10620
    Locus tag
    b0088
    See related
    ASAP:ABE-0000318
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0089
    Summary
    A carbamylated lysine residue is present in the active site. [More information is available at EcoGene: EG10620]. UDP-N-acetylmuramoylalanine-D-glutamate ligase catalyzes the addition of the second amino acid to the peptide moiety of the monomer unit of peptidoglycan. [More information is available at EcoCyc: EG10620].
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    Genomic context

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    Sequence:
    NC_000913.3 (97087..98403)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene peptidoglycan DD-transpeptidase FtsI Neighboring gene UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Neighboring gene D-alanyl-D-alanine-adding enzyme Neighboring gene phospho-N-acetylmuramoyl-pentapeptide-transferase Neighboring gene peptidoglycan glycosyltransferase FtsW Neighboring gene N-acetylglucosaminyl transferase Neighboring gene UDP-N-acetylmuramate--L-alanine ligase Neighboring gene D-alanine--D-alanine ligase B

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Large-scale preparation, purification, and crystallization of UDP-N-acetylmuramoyl-L-alanine: D-glutamate ligase from Escherichia coli. Auger G, et al. Protein Expr Purif, 1998 Jun. PMID 9631510
    2. Comparison of the D-glutamate-adding enzymes from selected gram-positive and gram-negative bacteria. Walsh AW, et al. J Bacteriol, 1999 Sep. PMID 10464212, Free PMC Article
    3. Role of the ortholog and paralog amino acid invariants in the active site of the UDP-MurNAc-L-alanine:D-glutamate ligase (MurD). Bouhss A, et al. Biochemistry, 1999 Sep 21. PMID 10493791
    4. "Open" structures of MurD: domain movements and structural similarities with folylpolyglutamate synthetase. Bertrand JA, et al. J Mol Biol, 2000 Sep 1. PMID 10966819
    5. Phosphinate Inhibitors of the D-Glutamic Acid-Adding Enzyme of Peptidoglycan Biosynthesis. Tanner ME, et al. J Org Chem, 1996 Mar 8. PMID 11667046

    See all (44) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A detailed analysis provides insight into the events that lead to the closure of MurD and reveals that minor structural modifications contribute to major overall conformation alterations.
      Title: Crystallographic Study of Peptidoglycan Biosynthesis Enzyme MurD: Domain Movement Revisited.
    2. Evaluation of three possible reaction pathways leading to tetrahedral intermediate formation by MurD ligase.
      Title: MurD ligase from E. coli: Tetrahedral intermediate formation study by hybrid quantum mechanical/molecular mechanical replica path method.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Over-production, purification and properties of the uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli.
    4. Results report the high resolution crystal structures of MurD in complexes with two novel inhibitors designed to mimic the transition state of the reaction, which contain either the D-Glu or the L-Glu moiety.
      Title: Structural and functional characterization of enantiomeric glutamic acid derivatives as potential transition state analogue inhibitors of MurD ligase.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables UDP-N-acetylmuramoylalanine-D-glutamate ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables UDP-N-acetylmuramoylalanine-D-glutamate ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acid-amino acid ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase
    NP_414630.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      97087..98403
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414630.1 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414630.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A895NVS9
      Conserved Domains (1) summary
      PRK03806
      Location:1 → 438
      murD; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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