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    hypD Fe-(CN)2CO cofactor assembly scaffold protein HypD [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947189, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hypD
    Official Full Name
    Fe-(CN)2CO cofactor assembly scaffold protein HypD
    Primary source
    ECOCYC:EG10486
    Locus tag
    b2729
    See related
    ASAP:ABE-0008965
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2724; hydF
    Summary
    HypD is an accessory protein that is essential for synthesis of all three hydrogenase isoenzymes . [More information is available at EcoCyc: EG10486].
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    Genomic context

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    Sequence:
    NC_000913.3 (2852136..2853257)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 3 nickel incorporation protein HypA Neighboring gene hydrogenase isoenzymes nickel incorporation protein HypB Neighboring gene hydrogenase maturation factor HypC Neighboring gene carbamoyl dehydratase HypE Neighboring gene DNA-binding transcriptional activator FhlA Neighboring gene protein YgbA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The iron-sulfur-containing HypC-HypD scaffold complex of the [NiFe]-hydrogenase maturation machinery is an ATPase. Nutschan K, et al. FEBS Open Bio, 2019 Dec. PMID 31614069, Free PMC Article
    2. HypD is the scaffold protein for Fe-(CN)2CO cofactor assembly in [NiFe]-hydrogenase maturation. Stripp ST, et al. Biochemistry, 2013 May 14. PMID 23597401
    3. Hydrogen metabolism in Escherichia coli: biochemical and genetic evidence for a hydF gene. Sankar P, et al. J Bacteriol, 1988 Dec. PMID 3056901, Free PMC Article
    4. Properties of the [NiFe]-hydrogenase maturation protein HypD. Blokesch M, et al. FEBS Lett, 2006 Jul 24. PMID 16814778
    5. New insights into [FeFe] hydrogenase activation and maturase function. Kuchenreuther JM, et al. PLoS One, 2012. PMID 23049878, Free PMC Article

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. analysis of a cell-free approach for H-cluster synthesis and hydrogenase maturation by using separately expressed and purified HydE, HydF, and HydG
      Title: New insights into [FeFe] hydrogenase activation and maturase function.
    2. dynamic complex between the hydrogenase maturation proteins HypD, HypC, HypE and HypF, which is the site of ligand biosynthesis and attachment to the iron atom of the NiFe site in hydrogenase
      Title: The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables carbon monoxide binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables carbon monoxide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables iron ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in protein maturation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein maturation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein-containing complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein-containing complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Fe-(CN)2CO cofactor assembly scaffold protein HypD

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2852136..2853257
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417209.1 Fe-(CN)2CO cofactor assembly scaffold protein HypD [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417209.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376J219
      Conserved Domains (1) summary
      TIGR00075
      Location:2 → 369
      hypD; hydrogenase expression/formation protein HypD
    Nucleotide Protein
    Heading Accession and Version

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