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    ompG outer membrane porin G [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945889, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ompG
    Official Full Name
    outer membrane porin G
    Primary source
    ECOCYC:G6657
    Locus tag
    b1319
    See related
    ASAP:ABE-0004425
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1315
    Summary
    OmpG is an efficient, nonspecific channel for mono-, di, and trisaccharides with sizes less than 600 Daltons although liposome swelling assays have demonstrated that OmpG is capable of transporting large solutes . [More information is available at EcoCyc: G6657].
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    Genomic context

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    Sequence:
    NC_000913.3 (1381947..1382852)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene kojibiose phosphorylase Neighboring gene beta-phosphoglucomutase Neighboring gene pseudo Neighboring gene pseudo Neighboring gene DNA-binding transcriptional repressor YcjW Neighboring gene DUF463 domain-containing protein YcjX Neighboring gene DUF697 domain-containing inner membrane protein YcjF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Incorporation of outer membrane protein OmpG in lipid membranes: protein-lipid interactions and beta-barrel orientation. Anbazhagan V, et al. Biochemistry, 2008 Jun 10. PMID 18473482
    2. Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli. Hiller M, et al. Chembiochem, 2005 Sep. PMID 16138308
    3. Folding of a monomeric porin, OmpG, in detergent solution. Conlan S, et al. Biochemistry, 2003 Aug 12. PMID 12899633
    4. Projection structure of the monomeric porin OmpG at 6 A resolution. Behlau M, et al. J Mol Biol, 2001 Jan 5. PMID 11114248
    5. Biochemical and biophysical characterization of OmpG: A monomeric porin. Conlan S, et al. Biochemistry, 2000 Oct 3. PMID 11009596

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system.
      Title: Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system.
    2. Correlation of membrane protein conformational and functional dynamics.
      Title: Correlation of membrane protein conformational and functional dynamics.
    3. A pH-independent quiet OmpG pore with enhanced electrostatic repulsion among the extracellular loops.
      Title: A pH-independent quiet OmpG pore with enhanced electrostatic repulsion among the extracellular loops.
    4. The X-ray structure of the alanine-substituted OmpG mutant obtained at pH 6.5 confirms the constitutively open conformation.
      Title: Correlation between the OmpG secondary structure and its pH-dependent alterations monitored by FTIR.
    5. At acidic pH, the authors detected a pH-dependent interaction at loop L6. This interaction changed the (un)folding of loop L6 and of beta-strands 11 and 12, which connect loop L6.
      Title: pH-dependent interactions guide the folding and gate the transmembrane pore of the beta-barrel membrane protein OmpG.
    6. Upon lowering the pH to 5.0, the conformation of OmpG molecules changed to close the extracellular entrance of their channel.
      Title: pH-induced conformational change of the beta-barrel-forming protein OmpG reconstituted into native E. coli lipids.
    7. Molecular dynamics simulations were used to identify regions of OmpG implicated in the gating.
      Title: Outer membrane protein G: Engineering a quiet pore for biosensing.
    8. Complete backbone assignments were obtained for 234 of 280 residues based on CA, CB, and CO connection pathways determined from a series of TROSY-based 3D experiments at 800 MHz.
      Title: Structure of outer membrane protein G by solution NMR spectroscopy.
    9. N-terminus verified by Edman degradation on mature peptide
      Title: Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels.
    10. The architecture of the channel, together with previous biochemical and other data, suggests that OmpG may form a non-specific channel for the transport of larger oligosaccharides.
      Title: Crystal structure of the monomeric porin OmpG.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables maltose transporting porin activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oligosaccharide transporting porin activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables porin activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables porin activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within carbohydrate transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in maltose transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in oligosaccharide transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cell outer membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    part_of pore complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    outer membrane porin G

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1381947..1382852
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415835.1 outer membrane porin G [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415835.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5Y800
      Conserved Domains (1) summary
      pfam09381
      Location:24 → 299
      Porin_OmpG; Outer membrane protein G (OmpG)
    Nucleotide Protein
    Heading Accession and Version

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