U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    flu self recognizing antigen 43 (Ag43) autotransporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946540, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    flu
    Official Full Name
    self recognizing antigen 43 (Ag43) autotransporter
    Primary source
    ECOCYC:G7080
    Locus tag
    b2000
    See related
    ASAP:ABE-0006642
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    agn; agn43; ECK1993; yeeQ; yzzX
    Summary
    Flu (Ag43) can be glycosylated in non-K-12 strains. [More information is available at EcoGene: EG12686]. agn43 encodes a 1,039 amino acid product with a 52 amino acid N-terminal signal sequence. [More information is available at EcoCyc: G7080].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2071539..2074658)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene ncRNA Neighboring gene inner membrane protein YeeR Neighboring gene RadC-like JAB domain-containing protein YeeS Neighboring gene DUF987 domain-containing protein YeeT

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. flu, a metastable gene controlling surface properties of Escherichia coli. Diderichsen B. J Bacteriol, 1980 Feb. PMID 6102553, Free PMC Article
    2. Biological Trojan horse: Antigen 43 provides specific bacterial uptake and survival in human neutrophils. Fexby S, et al. Infect Immun, 2007 Jan. PMID 17030570, Free PMC Article
    3. Epigenetic gene regulation in the bacterial world. Casadesús J, et al. Microbiol Mol Biol Rev, 2006 Sep. PMID 16959970, Free PMC Article
    4. Antigen-43-mediated autoaggregation impairs motility in Escherichia coli. Ulett GC, et al. Microbiology (Reading), 2006 Jul. PMID 16804184
    5. The turn of the screw: variations of the abundant beta-solenoid motif in passenger domains of Type V secretory proteins. Kajava AV, et al. J Struct Biol, 2006 Aug. PMID 16765057

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural stability for surface display of antigen 43 and application to bacterial outer membrane vesicles production.
      Title: Structural stability for surface display of antigen 43 and application to bacterial outer membrane vesicles production.
    2. ppGpp mediates the growth phase-dependent regulation of agn43, a phase variable gene, by stimulating its promoter activity.
      Title: ppGpp mediates the growth phase-dependent regulation of agn43, a phase variable gene, by stimulating its promoter activity.
    3. In the K-12 leader sequence, two in-frame translation initiation codons have been identified, one upstream and the other downstream of the transcription terminator. For optimal agn43 expression, both codons need to be present.
      Title: Control of gene expression at a bacterial leader RNA, the agn43 gene encoding outer membrane protein Ag43 of Escherichia coli.
    4. Lrp and MqsR, previously identified as agn43 regulators, do not regulate agn43 expression or ON/OFF switch frequency.
      Title: Did I pick the right colony? Pitfalls in the study of regulation of the phase variable antigen 43 adhesin.
    5. The data support a model in which in an ON-phase cell, the subcellular OxyR availability at the replication fork as it passes through the agn43 regulatory region is key for initiating an ON-to-OFF switch.
      Title: Establishing and maintaining sequestration of Dam target sites for phase variation of agn43 in Escherichia coli.
    6. The authors show that Ag43 mediates bacterial adhesion in a cell line-specific manner and that structural variations of the alpha(43) domain correlate with increased adhesive properties to proteins of the extracellular matrix such as collagen and laminin.
      Title: Impact of O-glycosylation on the molecular and cellular adhesion properties of the Escherichia coli autotransporter protein Ag43.
    7. Proposed roles of Ag43 expression are summarized and the studies are put into perspective regarding the role of allelic variants, genetic background of the bacterial strain, and control of expression by phase variation. Review.
      Title: Regulation and function of Ag43 (flu).
    8. that glycosylation may play significant roles in structural and functional properties of bacterial autotransporters Ag43 at many different levels.
      Title: Effect of glycosylation on the extracellular domain of the Ag43 bacterial autotransporter: enhanced stability and reduced cellular aggregation.
    9. In vivo, the H107A substitution in sigma(32) impedes recovery from heat shock (exposure to 42 degrees C), and it also leads to overexpression at lower temperatures (30 degrees C) of the Flu protein, which is associated with biofilm formation
      Title: Substitution of a highly conserved histidine in the Escherichia coli heat shock transcription factor, sigma32, affects promoter utilization in vitro and leads to overexpression of the biofilm-associated flu protein in vivo.
    10. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Component Evidence Code Pubs
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cell surface IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    self recognizing antigen 43 (Ag43) autotransporter

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2071539..2074658
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. YP_026164.1 self recognizing antigen 43 (Ag43) autotransporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for YP_026164.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376RGP0
      Conserved Domains (2) summary
      cd01344
      Location:517 → 707
      PL2_Passenger_AT; Pertactin-like passenger domains (virulence factors), C-terminal, subgroup 2, of autotransporter proteins of the type V secretion system of Gram-negative bacteria. This subgroup includes the passenger domains of the nonprotease autotransporters, Ag43, ...
      NF033177
      Location:1 → 1039
      auto_Ag43; autotransporter adhesin Ag43
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.