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    rsgA ribosome small subunit-dependent GTPase A [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948674, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rsgA
    Official Full Name
    ribosome small subunit-dependent GTPase A
    Primary source
    ECOCYC:G7841
    Locus tag
    b4161
    See related
    ASAP:ABE-0013626
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4157; yjeQ
    Summary
    rsgA(yjeQ) has been reported to be an essential gene, but deletion mutants have also been reported to grow slowly. [More information is available at EcoGene: EG12479]. Based on genetic interactions, RgsA/YjeQ is thought to play a role in late ribosome biogenesis . [More information is available at EcoCyc: G7841].
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    Genomic context

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    Sequence:
    NC_000913.3 (4390457..4391509, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene miniconductance mechanosensitive channel MscM Neighboring gene phosphatidylserine decarboxylase proenzyme Neighboring gene oligoribonuclease Neighboring gene tRNA-Gly

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Ribosome-small-subunit-dependent GTPase interacts with tRNA-binding sites on the ribosome. Kimura T, et al. J Mol Biol, 2008 Aug 29. PMID 18588897
    2. Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function. Campbell TL, et al. Biochem J, 2005 Aug 1. PMID 15828870, Free PMC Article
    3. Non-ribosomal factors in ribosome subunit assembly are emerging targets for new antibacterial drugs. Comartin DJ, et al. Curr Opin Pharmacol, 2006 Oct. PMID 16890019
    4. Interaction between RsgA and the ribosome. Kimura T, et al. Nucleic Acids Symp Ser (Oxf), 2007. PMID 18029743
    5. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. Hase Y, et al. RNA, 2009 Sep. PMID 19620234, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This finding indicates that, along with acting as an assembly factor, YjeQ has a role as a checkpoint protein, consisting of testing the proofreading ability of the 30S subunit. The structure also informs the mechanism by which YjeQ implements the release from the 30S subunit of a second assembly factor, called RbfA.
      Title: The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly.
    2. Results shows that YjeQ promotes the release of RbfA through the C-terminal helix in its zinc-finger domain.
      Title: The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly.
    3. costructure revealed that YjeQ interacts simultaneously with helix 44, the head and the platform of the 30S subunit
      Title: Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor.
    4. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells.
      Title: Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells.
    5. The work is most consistent with the hypothesis that YjeQ has a role in late 30S subunit biogenesis.
      Title: Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis.
    6. RsgA may be close contact with the A site of the decoding center of the small subunit of the ribosome.
      Title: Interaction between RsgA and the ribosome.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables GDP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within ribosomal small subunit assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosomal small subunit biogenesis EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in ribosomal small subunit biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within ribosomal small subunit biogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ribosome small subunit-dependent GTPase A
    NP_418585.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4390457..4391509 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418585.4 ribosome small subunit-dependent GTPase A [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418585.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7H9SCA2
      Conserved Domains (1) summary
      PRK12288
      Location:1 → 350
      PRK12288; small ribosomal subunit biogenesis GTPase RsgA
    Nucleotide Protein
    Heading Accession and Version

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