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    Med27 mediator complex subunit 27 [ Mus musculus (house mouse) ]

    Gene ID: 68975, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Med27provided by MGI
    Official Full Name
    mediator complex subunit 27provided by MGI
    Primary source
    MGI:MGI:1916225
    See related
    Ensembl:ENSMUSG00000026799 AllianceGenome:MGI:1916225
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Crsp8; D2Ertd434e; 1500015J03Rik; 2310042P07Rik
    Summary
    Predicted to enable transcription coactivator activity. Predicted to contribute to ubiquitin protein ligase activity. Acts upstream of or within somatic stem cell population maintenance. Predicted to be located in cytosol; nucleolus; and nucleoplasm. Predicted to be part of core mediator complex; mediator complex; and ubiquitin ligase complex. Is expressed in genitourinary system and tibialis anterior. Orthologous to human MED27 (mediator complex subunit 27). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Ubiquitous expression in whole brain E14.5 (RPKM 6.7), CNS E14 (RPKM 6.0) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Med27 in Genome Data Viewer
    Location:
    2 B; 2 19.84 cM
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (29236822..29414802)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (29346810..29524790)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:28980423-28980610 Neighboring gene STARR-positive B cell enhancer ABC_E5902 Neighboring gene STARR-seq mESC enhancer starr_04042 Neighboring gene STARR-seq mESC enhancer starr_04043 Neighboring gene senataxin Neighboring gene STARR-seq mESC enhancer starr_04046 Neighboring gene RIKEN cDNA 6530402F18 gene Neighboring gene netrin G2 Neighboring gene STARR-seq mESC enhancer starr_04048 Neighboring gene STARR-seq mESC enhancer starr_04049 Neighboring gene STARR-positive B cell enhancer ABC_E4423 Neighboring gene STARR-seq mESC enhancer starr_04051 Neighboring gene STARR-seq mESC enhancer starr_04052 Neighboring gene microRNA 133c Neighboring gene STARR-seq mESC enhancer starr_04054 Neighboring gene STARR-positive B cell enhancer ABC_E7802 Neighboring gene predicted gene, 30761 Neighboring gene STARR-seq mESC enhancer starr_04055 Neighboring gene STARR-positive B cell enhancer ABC_E9194 Neighboring gene STARR-positive B cell enhancer ABC_E2607 Neighboring gene STARR-seq mESC enhancer starr_04057 Neighboring gene Rap guanine nucleotide exchange factor (GEF) 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:29526317-29526500 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:29529743-29529852 Neighboring gene STARR-seq mESC enhancer starr_04064 Neighboring gene STARR-positive B cell enhancer ABC_E9506 Neighboring gene STARR-positive B cell enhancer ABC_E2608 Neighboring gene STARR-seq mESC enhancer starr_04069

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The essential role of MED27 in stabilizing the mediator complex for cardiac development and function. Zhu S, et al. Life Sci, 2024 Nov 1. PMID 39209248
    2. Cloning of wrinkle-free, a previously uncharacterized mouse mutation, reveals crucial roles for fatty acid transport protein 4 in skin and hair development. Moulson CL, et al. Proc Natl Acad Sci U S A, 2003 Apr 29. PMID 12697906, Free PMC Article
    3. Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Kolonay DW, et al. Front Cell Dev Biol, 2024. PMID 38690566, Free PMC Article
    4. Novel critical role of a human Mediator complex for basal RNA polymerase II transcription. Mittler G, et al. EMBO Rep, 2001 Sep. PMID 11559591, Free PMC Article
    5. Mediator and cohesin connect gene expression and chromatin architecture. Kagey MH, et al. Nature, 2010 Sep 23. PMID 20720539, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The essential role of MED27 in stabilizing the mediator complex for cardiac development and function.
      Title: The essential role of MED27 in stabilizing the mediator complex for cardiac development and function.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables transcription coactivator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coactivator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transcription coactivator activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to ubiquitin protein ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein ubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within somatic stem cell population maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of core mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleolus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    part_of transcription regulator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of ubiquitin ligase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of ubiquitin ligase complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 27
    Names
    CRSP complex subunit 8
    cofactor required for Sp1 transcriptional activation, subunit 8
    transcriptional co-activator CRSP8 homolog

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001290489.1 → NP_001277418.1  mediator of RNA polymerase II transcription subunit 27 isoform c

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) lacks two 5' exons but instead contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a.
      Source sequence(s)
      AI850753, AL772213, AL929437, CJ073494
      Consensus CDS
      CCDS79764.1
      UniProtKB/TrEMBL
      E0CZG8
      Related
      ENSMUSP00000125390.2, ENSMUST00000159280.8
      Conserved Domains (1) summary
      pfam11571
      Location:89 → 170
      Med27; Mediator complex subunit 27

    2. NM_001290490.1 → NP_001277419.1  mediator of RNA polymerase II transcription subunit 27 isoform d

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) contains alternate 5' and 3' exon structure, and it thus differs in both UTRs and in the 5' and 3' coding regions, compared to variant 1. The encoded isoform (d) has distinct N- and C-termini and is shorter than isoform a.
      Source sequence(s)
      AK147181, AL929437, CJ073494, DV648756
      UniProtKB/Swiss-Prot
      Q9DB40

    3. NM_026896.5 → NP_081172.2  mediator of RNA polymerase II transcription subunit 27 isoform a

      See identical proteins and their annotated locations for NP_081172.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (a).
      Source sequence(s)
      AI850753, AK005251, CJ072886
      Consensus CDS
      CCDS15853.1
      UniProtKB/Swiss-Prot
      A2AJV2, Q78JI3, Q9D707, Q9DB40
      Related
      ENSMUSP00000028139.5, ENSMUST00000028139.11
      Conserved Domains (1) summary
      pfam11571
      Location:228 → 306
      Med27; Mediator complex subunit 27

    4. NM_029615.1 → NP_083891.1  mediator of RNA polymerase II transcription subunit 27 isoform b

      See identical proteins and their annotated locations for NP_083891.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks four 3' exons but instead contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a.
      Source sequence(s)
      AK009764, AK147181, AL929437, CJ072886
      Consensus CDS
      CCDS71014.1
      UniProtKB/Swiss-Prot
      Q9DB40
      Related
      ENSMUSP00000109461.5, ENSMUST00000113830.11

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      29236822..29414802
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017319247.3 → XP_017174736.1  mediator of RNA polymerase II transcription subunit 27 isoform X2

    2. XM_017319246.1 → XP_017174735.1  mediator of RNA polymerase II transcription subunit 27 isoform X1

      Conserved Domains (1) summary
      pfam11571
      Location:115 → 194
      Med27; Mediator complex subunit 27
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9DB40.2 GenPept UniProtKB/Swiss-Prot:Q9DB40

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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