Eno1 enolase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Eno1provided by RGD
Official Full Name: enolase 1provided by RGD
Primary source: RGD:2553
See related: EnsemblRapid:ENSRNOG00000017895 AllianceGenome:RGD:2553
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Nne
Summary: Enables several functions, including heat shock protein binding activity; phosphopyruvate hydratase activity; and protein homodimerization activity. Involved in ERK1 and ERK2 cascade; canonical glycolysis; and cellular response to hypoxia. Located in several cellular components, including growth cone; membrane raft; and synaptic membrane. Part of phosphopyruvate hydratase complex. Biomarker of hypertension. Human ortholog(s) of this gene implicated in Alzheimer's disease. Orthologous to human ENO1 (enolase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 3105.7), Thymus (RPKM 1709.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 5q36

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	5	NC_086023.1 (166002867..166014252)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	5	NC_051340.1 (160719951..160731337)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	5	NC_005104.4 (167288223..167299610)

Chromosome 5 - NC_086023.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Molecular characterization of a 1p36 chromosomal duplication and in utero interference define ENO1 as a candidate gene for polymicrogyria.
Title: Molecular characterization of a 1p36 chromosomal duplication and in utero interference define ENO1 as a candidate gene for polymicrogyria.
CircZNF609 aggravates neuropathic pain via miR-22-3p/ENO1 axis in CCI rat models.
Title: CircZNF609 aggravates neuropathic pain via miR-22-3p/ENO1 axis in CCI rat models.
SIRT1 may regulate acetylation level of NSE and PKM through deacetylation and further regulate their catalytic activity.
Title: SIRT 1 binding with PKM and NSE and modulate their acetylation and activities.
regulates the metabolic reprogramming and malignant phenotype of pulmonary artery smooth muscle cells in pulmonary arterial hypertension
Title: Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway.
gamma-enolase may signal and correlate lung tissue damage well before obvious physical pathological tissue damage and might be a candidate diagnostic and/or prognostic marker.
Title: Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.
The present study aimed to investigate the change of alpha-enolase during cardiac hypertrophy and explore its role in this pathological process.
Title: Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.
alpha-enolase has a catalytically independent role in inducing cardiomyocyte apoptosis and mitochondrial dysfunction.
Title: α-Enolase plays a catalytically independent role in doxorubicin-induced cardiomyocyte apoptosis and mitochondrial dysfunction.
this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase.
Title: Constitutive heat shock protein 70 interacts with α-enolase and protects cardiomyocytes against oxidative stress.
alpha-Enolase binds to RNA
Title: α-Enolase binds to RNA.
Data show that the expression of alpha-enolase in the left ventricle of the rats during normal and pathological cardiac development is different.
Title: Differential expression of alpha-enolase in the normal and pathological cardiac growth.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MARC_24023-24024:1029959245:1 (e-PCR)
- UniSTS:268830

RH128457 (e-PCR)
- UniSTS:211765

RH94671 (e-PCR)
- UniSTS:119918

RH125429 (e-PCR)
- UniSTS:162251

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables GTPase binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables heat shock protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables phosphopyruvate hydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphopyruvate hydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphopyruvate hydratase activity	IEA Inferred from Electronic Annotation more info
enables phosphopyruvate hydratase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info
enables transcription corepressor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ERK1 and ERK2 cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in canonical glycolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in canonical glycolysis	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to interleukin-7	ISO Inferred from Sequence Orthology more info
involved_in gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within in utero embryonic development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ATP biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of plasminogen activation	ISO Inferred from Sequence Orthology more info
involved_in response to virus	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear outer membrane	ISO Inferred from Sequence Orthology more info
part_of phosphopyruvate hydratase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphopyruvate hydratase complex	IC Inferred by Curator more info	PubMed
part_of phosphopyruvate hydratase complex	IDA Inferred from Direct Assay more info	PubMed
part_of phosphopyruvate hydratase complex	IEA Inferred from Electronic Annotation more info
part_of phosphopyruvate hydratase complex	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: alpha-enolase

Names: 2-phospho-D-glycerate hydro-lyase; enolase 1, (alpha); enolase 1, alpha non-neuron; non-neural enolase

NP_001103378.1

EC 4.2.1.11

NP_036686.2

EC 4.2.1.11

XP_006239506.1

EC 4.2.1.11

XP_038965185.1

EC 4.2.1.11

XP_038965186.1

EC 4.2.1.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001109908.2 → NP_001103378.1 alpha-enolase isoform 1

See identical proteins and their annotated locations for NP_001103378.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93

Related

ENSRNOP00000073191.1, ENSRNOT00000081579.3

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase
NM_001429239.1 → NP_001416168.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429240.1 → NP_001416169.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429241.1 → NP_001416170.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429242.1 → NP_001416171.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429243.1 → NP_001416172.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429244.1 → NP_001416173.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429245.1 → NP_001416174.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93
NM_001429246.1 → NP_001416175.1 alpha-enolase isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/TrEMBL

A0A8I6ART9, M0R5J4
NM_001429248.1 → NP_001416177.1 alpha-enolase isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/TrEMBL

A0A8L2QCV1, M0R5J4
NM_012554.4 → NP_036686.2 alpha-enolase isoform 1

See identical proteins and their annotated locations for NP_036686.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

P04764, Q66HI3, Q6AYV3, Q6P504

UniProtKB/TrEMBL

A0A9K3Y892, M0R5J4, Q4QR91, Q5BJ93

Related

ENSRNOP00000024106.6, ENSRNOT00000024106.9

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086023.1 Reference GRCr8

Range

166002867..166014252

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039109257.2 → XP_038965185.1 alpha-enolase isoform X2

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase
XM_039109258.2 → XP_038965186.1 alpha-enolase isoform X2

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase
XM_006239444.4 → XP_006239506.1 alpha-enolase isoform X1

Related

ENSRNOP00000096876.1, ENSRNOT00000119075.2

Conserved Domains (2) summary

PLN00191
Location:2 → 432

PLN00191; enolase

cd03313
Location:5 → 416

enolase; Enolase: Enolases are homodimeric enzymes that catalyse the reversible dehydration of 2-phospho-D-glycerate to phosphoenolpyruvate as part of the glycolytic and gluconeogenesis pathways. The reaction is facilitated by the presence of metal ions.