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    pilA type 4 fimbrial protein PilA [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 878423, updated on 24-Aug-2024

    Summary

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    Gene symbol
    pilA
    Gene description
    type 4 fimbrial protein PilA
    Locus tag
    PA4525
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (5069082..5069531, complement)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene nicotinate-nucleotide pyrophosphorylase Neighboring gene tRNA-Thr Neighboring gene type 4 fimbrial biogenesis protein PilB Neighboring gene pseudo

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Pseudomonas aeruginosa defends against phages through type IV pilus glycosylation. Harvey H, et al. Nat Microbiol, 2018 Jan. PMID 29133883
    2. Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene. Pasloske BL, et al. FEBS Lett, 1985 Apr 22. PMID 2985436
    3. Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa. Alm RA, et al. Gene, 1997 Jun 11. PMID 9224878
    4. Twitching motility contributes to the role of pili in corneal infection caused by Pseudomonas aeruginosa. Zolfaghar I, et al. Infect Immun, 2003 Sep. PMID 12933890, Free PMC Article
    5. Involvement of bacterial migration in the development of complex multicellular structures in Pseudomonas aeruginosa biofilms. Klausen M, et al. Mol Microbiol, 2003 Oct. PMID 14507363

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Glycosylation of type 4 fimbrial protein (PilA) protect Pseudomonas aeruginosa from certain pilus-specific phages. Alterations to PilA sequence can block phage infection, but glycosylation is less likely to create disadvantageous phenotypes. Through construction of chimeric phages, specific phage tail proteins allow infection of P. aeruginosa strains with glycosylated pili.
      Title: Pseudomonas aeruginosa defends against phages through type IV pilus glycosylation.
    2. PilS's phosphatase motif was required for the autoregulation of pilA transcription, suggesting that under conditions where PilA is abundant, the PilA-PilS interaction promotes PilR dephosphorylation and thus down-regulation of further pilA transcription.
      Title: Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS.
    3. Data show that the type IV pilus (Tfp)-deficient mutant DeltapilA is severely attenuated in an acute pneumonia model of infection in the lungs of wild-type mice, but is virulent in the lungs of surfactant protein-A SP-A(-/-) mice.
      Title: Type IV pilus of Pseudomonas aeruginosa confers resistance to antimicrobial activities of the pulmonary surfactant protein-A.
    4. Found that the calcium-modulating cyclophilin ligand (CAMLG), which is involved in Ca(2+) signaling, was the major host cell PilA binding protein.
      Title: Type IV pilus protein PilA of Pseudomonas aeruginosa modulates calcium signaling through binding the calcium-modulating cyclophilin ligand.
    5. PilA, the major pilin subunit, interacts with PilNOPQ. The results allow us to propose a model whereby PilA makes extensive contacts with the transenvelope complex, possibly to increase local concentrations of PilA monomers for polymerization.
      Title: PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by PseudoCAP

    Process Evidence Code Pubs
    acts_upstream_of_or_within cell adhesion involved in single-species biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of calcium-mediated signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within single-species biofilm formation on inanimate substrate IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within type IV pilus-dependent motility IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within type IV pilus-dependent motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in pilus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in type IV pilus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    type 4 fimbrial protein PilA
    NP_253215.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      5069082..5069531 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_253215.1 type 4 fimbrial protein PilA [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_253215.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      Q93PL8
      Conserved Domains (1) summary
      COG4969
      Location:1 → 134
      PilA; Tfp pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P04739.1

    Gene LinkOut

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    Molecular Biology Databases
    Research Materials