Sh3rf1 SH3 domain containing ring finger 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sh3rf1provided by MGI
Official Full Name: SH3 domain containing ring finger 1provided by MGI
Primary source: MGI:MGI:1913066
See related: Ensembl:ENSMUSG00000031642 AllianceGenome:MGI:1913066
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Posh; Sh3md2; 2200003J05Rik
Summary: Enables MAP-kinase scaffold activity. Involved in neuron migration; positive regulation of JNK cascade; and regulation of alpha-beta T cell activation. Located in lamellipodium. Is expressed in several structures, including central nervous system and genitourinary system. Orthologous to human SH3RF1 (SH3 domain containing ring finger 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in whole brain E14.5 (RPKM 9.0), lung adult (RPKM 8.7) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 B3.1; 8 30.94 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (61677117..61918327)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (61224083..61465293)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.
Title: POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.
The pro-apoptotic JNK scaffold protein POSH mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia.
Title: The pro-apoptotic JNK scaffold POSH/SH3RF1 mediates CHMP2BIntron5-associated toxicity in animal models of frontotemporal dementia.
this study shows that POSH is essential for the integration of cell-type-specific signals that regulate the differentiation, survival, and function of T cells
Title: POSH Regulates CD4+ T Cell Differentiation and Survival.
identify plenty of SH3 (POSH) and JNK-interacting protein 1 (JIP-1) as a multiprotein scaffold network for TCR-mediated JNK1 activation in CD8(+) T cells.
Title: The POSH/JIP-1 scaffold network regulates TCR-mediated JNK1 signals and effector function in CD8(+) T cells.
a mechanism by which POSH regulates the localization of activated Rac1 and F-actin assembly to control PCDLP formation and subsequent somal translocation of migratory neurons.
Title: POSH localizes activated Rac1 to control the formation of cytoplasmic dilation of the leading process and neuronal migration.
An intracellular signaling pathway for process outgrowth inhibition by Nogo66 implicates the POSH complex as a therapeutic target to enhance axon outgrowth and plasticity in the injured central nervous system.
Title: POSH is an intracellular signal transducer for the axon outgrowth inhibitor Nogo66.
Results suggest that the molecular scaffold protein POSH assembles an inhibitory complex that links to the actin-myosin network to regulate neuronal process outgrowth.
Title: The scaffold protein POSH regulates axon outgrowth.
POSH and JIPs directly associate with one another to form a multiprotein complex, PJAC (POSH-JIP apoptotic complex), that includes all of the known kinase components of the pathway.
Title: Direct interaction of the molecular scaffolds POSH and JIP is required for apoptotic activation of JNKs.
Siah1 is a binding partner for POSH (plenty of SH3s), a scaffold component of the apoptotic JNK pathway
Title: Siah1 interacts with the scaffold protein POSH to promote JNK activation and apoptosis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8Mit128 (e-PCR), detects polymorphism
- UniSTS:131331

RH125258 (e-PCR)
- UniSTS:162080

R75531 (e-PCR)
- UniSTS:179746

D8Mit160 (e-PCR), detects polymorphism
- UniSTS:131365

AF030131 (e-PCR)
- UniSTS:163438

NoName (e-PCR)
- UniSTS:224689

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables MAP-kinase scaffold activity	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of JNK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of JNK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein autoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein autoubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of CD4-positive, alpha-beta T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of CD8-positive, alpha-beta T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase SH3RF1

Names: RING-type E3 ubiquitin transferase SH3RF1; SH3 domain-containing RING finger protein 1; SH3 multiple domains 2; SH3 multiple domains protein 2; plenty of SH3 domains; plenty of SH3s; putative E3 ubiquitin-protein ligase SH3RF1

NP_067481.2

EC 2.3.2.27

XP_011240533.1

EC 2.3.2.27

XP_036010097.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021506.3 → NP_067481.2 E3 ubiquitin-protein ligase SH3RF1

Status: VALIDATED

Source sequence(s)

AC117196, AC134339

Consensus CDS

CCDS40348.1

UniProtKB/Swiss-Prot

O70254, Q3UG42, Q69ZI1, Q6P9M8, Q8BR66, Q8C2T5

UniProtKB/TrEMBL

E9QQ33

Related

ENSMUSP00000034060.6, ENSMUST00000034060.7

Conserved Domains (5) summary

cd11785
Location:836 → 890

SH3_SH3RF_C; C-terminal (Fourth) Src Homology 3 domain of SH3 domain containing ring finger 1 (SH3RF1), SH3RF3, and similar domains

cd11930
Location:200 → 254

SH3_SH3RF1_2; Second Src Homology 3 domain of SH3 domain containing ring finger protein 1, an E3 ubiquitin-protein ligase

PHA03247
Location:539 → 851

PHA03247; large tegument protein UL36; Provisional

cd16748
Location:8 → 55

RING-HC_SH3RF1; RING finger, HC subclass, found in SH3 domain-containing RING finger protein 1 (SH3RF1) and similar proteins

cl17036
Location:455 → 509

SH3; Src Homology 3 domain superfamily

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

61677117..61918327

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242231.2 → XP_011240533.1 E3 ubiquitin-protein ligase SH3RF1 isoform X2

UniProtKB/TrEMBL

A0A1B0GSY0

Related

ENSMUSP00000148118.2, ENSMUST00000209611.2

Conserved Domains (5) summary

cd11930
Location:200 → 254

SH3_SH3RF1_2; Second Src Homology 3 domain of SH3 domain containing ring finger protein 1, an E3 ubiquitin-protein ligase

PHA03247
Location:539 → 832

PHA03247; large tegument protein UL36; Provisional

cd00162
Location:12 → 52

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cl17036
Location:455 → 509

SH3; Src Homology 3 domain superfamily

cl17238
Location:8 → 55

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_036154204.1 → XP_036010097.1 E3 ubiquitin-protein ligase SH3RF1 isoform X1

UniProtKB/Swiss-Prot

O70254, Q3UG42, Q69ZI1, Q6P9M8, Q8BR66, Q8C2T5

UniProtKB/TrEMBL

E9QQ33

Conserved Domains (5) summary

cd11785
Location:836 → 890

SH3_SH3RF_C; C-terminal (Fourth) Src Homology 3 domain of SH3 domain containing ring finger 1 (SH3RF1), SH3RF3, and similar domains

cd11930
Location:200 → 254

SH3_SH3RF1_2; Second Src Homology 3 domain of SH3 domain containing ring finger protein 1, an E3 ubiquitin-protein ligase

PHA03247
Location:539 → 851

PHA03247; large tegument protein UL36; Provisional

cd16748
Location:8 → 55

RING-HC_SH3RF1; RING finger, HC subclass, found in SH3 domain-containing RING finger protein 1 (SH3RF1) and similar proteins

cl17036
Location:455 → 509

SH3; Src Homology 3 domain superfamily

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_198678.1: Suppressed sequence

Description

NM_198678.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.