Naip5 NLR family, apoptosis inhibitory protein 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Naip5provided by MGI
Official Full Name: NLR family, apoptosis inhibitory protein 5provided by MGI
Primary source: MGI:MGI:1298220
See related: Ensembl:ENSMUSG00000071203 AllianceGenome:MGI:1298220
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lgn1; Birc1e; Naip-rs3
Summary: Enables ATP binding activity. Involved in positive regulation of interleukin-1 beta production; pyroptotic inflammatory response; and response to bacterium. Acts upstream of or within symbiont entry into host cell. Part of IPAF inflammasome complex. Is expressed in several structures, including central nervous system; genitourinary system; heart; liver; and retina. Orthologous to human NAIP (NLR family apoptosis inhibitory protein). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in large intestine adult (RPKM 25.6), small intestine adult (RPKM 11.9) and 8 other tissues See more
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Genomic context

Location:: 13 D1; 13 53.05 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (100348243..100387437, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (100211735..100250930, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Six distinct NAIP5 domains contact multiple conserved regions of flagellin, prying NAIP5 into an open and active conformation.
Title: The structural basis of flagellin detection by NAIP5: A strategy to limit pathogen immune evasion.
These results provide definitive genetic evidence for the important physiological function of NAIPs in antibacterial defense and inflammatory damage-induced lethality in mice.
Title: Genetic functions of the NAIP family of inflammasome receptors for bacterial ligands in mice.
Arsenicals, including arsenic trioxide and sodium arsenite, inhibited activation of the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes.
Title: Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes.
Primary microglial cells have a functional Naip5-NLR family CARD domain containing 4 (NLRC4) inflammasome likely to be important to monitor and clear central nervous system infections by flagellated bacteria.
Title: Inflammasome activation restricts Legionella pneumophila replication in primary microglial cells through flagellin detection.
the NAIP5-NLRC4 inflammasome is induced by direct interactions with conserved N- and C-terminal regions of flagellin
Title: Formation and structure of a NAIP5-NLRC4 inflammasome induced by direct interactions with conserved N- and C-terminal regions of flagellin.
genetic polymorphism is associated with susceptibility to Legionella pneumophila infection
Title: Global cellular changes induced by Legionella pneumophila infection of bone marrow-derived macrophages.
Our results indicate that NAIP5 regulates the apparent specificity of the NLRC4 inflammasome for distinct bacterial ligands.
Title: Differential requirements for NAIP5 in activation of the NLRC4 inflammasome.
Data define an additional anti-pathogen effector mechanism operated through Naip5 and Nlrc4 inflammasomes and illustrated a novel signaling transduction pathway that activates iNOS.
Title: A novel pathway for inducible nitric-oxide synthase activation through inflammasomes.
flagellin sensing by the NLR proteins Naip5 and Nlrc4 may be coupled to Irf1-Irf8-mediated transcriptional activation of key effector genes essential for macrophage resistance to L. pneumophila infection.
Title: Restriction of Legionella pneumophila replication in macrophages requires concerted action of the transcriptional regulators Irf1 and Irf8 and nod-like receptors Naip5 and Nlrc4.
mice deficient in the intracellular sensor Naip5 failed to activate the inflammasome in response to the 35 amino acids of flagellin or in response to Legionella pneumophila infection.
Title: Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (2)
Endonuclease-mediated (1)
Spontaneous (1) 1 citation
Targeted (2) 1 citation

General gene information

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Markers

Naip1 (e-PCR), detects polymorphism
- UniSTS:534557

D13Die34 (e-PCR), detects polymorphism
- UniSTS:164905

D13Die25 (e-PCR), detects polymorphism
- UniSTS:141911

D13Die28 (e-PCR), detects polymorphism
- UniSTS:141914

Birc1e (e-PCR), detects polymorphism
- UniSTS:256362

D13Die31a (e-PCR), detects polymorphism
- UniSTS:186736

D13Mit37a (e-PCR), detects polymorphism
- UniSTS:141938

Birc1-rs1 (e-PCR), detects polymorphism
- UniSTS:143986

Birc1-rs1 (e-PCR), detects polymorphism
- UniSTS:143987

D13Die35a (e-PCR), detects polymorphism
- UniSTS:186739

Naip1 (e-PCR), detects polymorphism
- UniSTS:496020

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to Gram-negative bacterium	TAS Traceable Author Statement more info	PubMed
involved_in defense response to bacterium	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of bacterium	IBA Inferred from Biological aspect of Ancestor more info
involved_in detection of bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in innate immune response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of JNK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-1 beta production	IDA Inferred from Direct Assay more info	PubMed
involved_in pyroptotic inflammatory response	IBA Inferred from Biological aspect of Ancestor more info
involved_in pyroptotic inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_negative_effect regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within symbiont entry into host cell	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within symbiont entry into host cell	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of IPAF inflammasome complex	IBA Inferred from Biological aspect of Ancestor more info
part_of IPAF inflammasome complex	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in perikaryon	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: baculoviral IAP repeat-containing protein 1e

Names: Legionella, susceptiblility 1; baculoviral IAP repeat-containing 1e; neuronal apoptosis inhibitory protein 5; neuronal apoptosis inhibitory protein, related sequence 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001426264.1 → NP_001413193.1 baculoviral IAP repeat-containing protein 1e isoform 1

Status: VALIDATED

Source sequence(s)

CT009518, CT030167

UniProtKB/Swiss-Prot

O09121, O09122, P81703, Q8CGT2, Q9R016, Q9R029
NM_001426265.1 → NP_001413194.1 baculoviral IAP repeat-containing protein 1e isoform 2

Status: VALIDATED

Source sequence(s)

CT009518, CT030167
NM_010870.3 → NP_035000.2 baculoviral IAP repeat-containing protein 1e isoform 1

See identical proteins and their annotated locations for NP_035000.2

Status: VALIDATED

Source sequence(s)

CT009518, CT030167

Consensus CDS

CCDS26727.1

UniProtKB/Swiss-Prot

O09121, O09122, P81703, Q8CGT2, Q9R016, Q9R029

UniProtKB/TrEMBL

Q8CGT3

Conserved Domains (8) summary

cd09293
Location:1072 → 1271

AMN1; Antagonist of mitotic exit network protein 1

cd00022
Location:279 → 346

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

sd00033
Location:1055 → 1078

LRR_RI; leucine-rich repeat [structural motif]

sd00034
Location:1132 → 1156

LRR_AMN1; leucine-rich repeat [structural motif]

pfam00653
Location:162 → 228

BIR; Inhibitor of Apoptosis domain

pfam05729
Location:464 → 618

NACHT; NACHT domain

pfam17779
Location:688 → 743

NOD2_WH; NOD2 winged helix domain

pfam17889
Location:767 → 873

NLRC4_HD; NLRC4 helical domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

100348243..100387437 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011244631.3 → XP_011242933.1 baculoviral IAP repeat-containing protein 1e isoform X2

UniProtKB/TrEMBL

Q8CGT3

Conserved Domains (8) summary

cd09293
Location:1026 → 1225

AMN1; Antagonist of mitotic exit network protein 1

cd00022
Location:233 → 300

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

sd00033
Location:1009 → 1032

LRR_RI; leucine-rich repeat [structural motif]

sd00034
Location:1086 → 1110

LRR_AMN1; leucine-rich repeat [structural motif]

pfam00653
Location:116 → 182

BIR; Inhibitor of Apoptosis domain

pfam05729
Location:418 → 572

NACHT; NACHT domain

pfam17779
Location:642 → 697

NOD2_WH; NOD2 winged helix domain

pfam17889
Location:721 → 827

NLRC4_HD; NLRC4 helical domain
XM_017315416.2 → XP_017170905.1 baculoviral IAP repeat-containing protein 1e isoform X3

UniProtKB/TrEMBL

Q8CGT3

Conserved Domains (6) summary

cd00022
Location:279 → 346

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

sd00033
Location:1055 → 1078

LRR_RI; leucine-rich repeat [structural motif]

pfam00653
Location:162 → 228

BIR; Inhibitor of Apoptosis domain

pfam05729
Location:464 → 618

NACHT; NACHT domain

pfam17779
Location:688 → 743

NOD2_WH; NOD2 winged helix domain

pfam17889
Location:767 → 873

NLRC4_HD; NLRC4 helical domain
XM_017315417.2 → XP_017170906.1 baculoviral IAP repeat-containing protein 1e isoform X5

UniProtKB/TrEMBL

Q8CGT3

Conserved Domains (7) summary

cd09293
Location:808 → 927

AMN1; Antagonist of mitotic exit network protein 1

cd00022
Location:15 → 82

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

sd00034
Location:868 → 892

LRR_AMN1; leucine-rich repeat [structural motif]

pfam05729
Location:200 → 354

NACHT; NACHT domain

pfam17779
Location:424 → 479

NOD2_WH; NOD2 winged helix domain

pfam17889
Location:503 → 609

NLRC4_HD; NLRC4 helical domain

cl39015
Location:799 → 1067

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...
XM_017315415.3 → XP_017170904.1 baculoviral IAP repeat-containing protein 1e isoform X1

UniProtKB/Swiss-Prot

O09121, O09122, P81703, Q8CGT2, Q9R016, Q9R029

UniProtKB/TrEMBL

Q8CGT3

Related

ENSMUSP00000058611.3, ENSMUST00000049789.3

Conserved Domains (8) summary

cd09293
Location:1072 → 1271

AMN1; Antagonist of mitotic exit network protein 1

cd00022
Location:279 → 346

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

sd00033
Location:1055 → 1078

LRR_RI; leucine-rich repeat [structural motif]

sd00034
Location:1132 → 1156

LRR_AMN1; leucine-rich repeat [structural motif]

pfam00653
Location:162 → 228

BIR; Inhibitor of Apoptosis domain

pfam05729
Location:464 → 618

NACHT; NACHT domain

pfam17779
Location:688 → 743

NOD2_WH; NOD2 winged helix domain

pfam17889
Location:767 → 873

NLRC4_HD; NLRC4 helical domain
XM_036157899.1 → XP_036013792.1 baculoviral IAP repeat-containing protein 1e isoform X4

UniProtKB/TrEMBL

Q8CGT3

Conserved Domains (7) summary

cd09293
Location:1072 → 1271

AMN1; Antagonist of mitotic exit network protein 1

cd00022
Location:279 → 346

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

sd00033
Location:1055 → 1078

LRR_RI; leucine-rich repeat [structural motif]

pfam00653
Location:162 → 228

BIR; Inhibitor of Apoptosis domain

pfam05729
Location:464 → 618

NACHT; NACHT domain

pfam17779
Location:688 → 743

NOD2_WH; NOD2 winged helix domain

pfam17889
Location:767 → 873

NLRC4_HD; NLRC4 helical domain