Ncs1 neuronal calcium sensor 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ncs1provided by RGD
Official Full Name: neuronal calcium sensor 1provided by RGD
Primary source: RGD:68417
See related: EnsemblRapid:ENSRNOG00000064701 AllianceGenome:RGD:68417
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Freq
Summary: Enables metal ion binding activity; protein kinase binding activity; and voltage-gated calcium channel activity. Involved in several processes, including positive regulation of calcium-mediated signaling; regulation of exocytosis; and regulation of presynaptic cytosolic calcium ion concentration. Located in dense core granule. Is active in several cellular components, including calyx of Held; postsynaptic cytosol; and presynaptic cytosol. Orthologous to human NCS1 (neuronal calcium sensor 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 361.8), Heart (RPKM 84.8) and 4 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3p12

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (34920949..34966554)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (14523220..14568829)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (10556250..10602672, complement)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

three critical amino acid residues of N. crassa NCS-1 and its functional conservation across species using the orthologue from R. norvegicus
Title: Identification of critical amino acid residues and functional conservation of the Neurospora crassa and Rattus norvegicus orthologues of neuronal calcium sensor-1.
In the Ca(2+)/NCS-1.D2R peptide complex, the C-terminal region adopts a 310 helix-turn-310 helix, whereas in the GRK1 peptide complex it forms an a-helix
Title: Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
NCS-1 was decreased in prefrontal cortex 48 hours after sepsis induction
Title: Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and prefrontal cortex in rats submitted to sepsis.
physical exercise could be modulating NCS-1 in an activity dependent manner, for specific neural substrates
Title: Differential effects of swimming training on neuronal calcium sensor-1 expression in rat hippocampus/cortex and in object recognition memory tasks.
NCS-1 modulates PKA/cAMP signaling pathway.
Title: Downregulation of the cAMP/PKA pathway in PC12 cells overexpressing NCS-1.
characterized individual residues in both non-myristoylated and myristoylated NCS-1 that access alternate states by measuring nonlinear temperature dependence of the backbone amide-proton ((1)H(N)) chemical shifts
Title: Characterization of low-energy excited states in the native state ensemble of non-myristoylated and myristoylated neuronal calcium sensor-1.
findings demonstrate that increasing the levels of intracellular NCS1 in injured and uninjured central neurons enhances their intrinsic anatomical plasticity within the injured adult central nervous system
Title: Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
Data show that overexpression of NCS-1 in PC12 cells induces an enhancement of intracellular second messenger and transmitter release dependent on CCH response, suggesting that muscarinic signaling is "up-regulated" in this cell model.
Title: Modulation of muscarinic signaling in PC12 cells overexpressing neuronal Ca2+ sensor-1 protein.
These data demonstrate a role for NCS-1 and dopamine type-2 receptor in dentate gyrus (DG) plasticity and provide insight for understanding how the DG contributes to the origin of exploration and spatial memory acquisition
Title: NCS-1 in the dentate gyrus promotes exploration, synaptic plasticity, and rapid acquisition of spatial memory.
This study demonstrates the importance of Mg(2+) as a modulator of calcium homeostasis and active-state behavior of NCS-1.
Title: Regulatory and structural EF-hand motifs of neuronal calcium sensor-1: Mg 2+ modulates Ca 2+ binding, Ca 2+ -induced conformational changes, and equilibrium unfolding transitions.

Submit: New GeneRIF Correction See all GeneRIFs (29)

General gene information

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Markers

D3Rat232 (e-PCR), detects polymorphism
- UniSTS:227617

AW531469 (e-PCR)
- UniSTS:245104

D3Rat194 (e-PCR), detects polymorphism
- UniSTS:227583

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium sensitive guanylate cyclase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent protein kinase inhibitor activity	TAS Traceable Author Statement more info	PubMed
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables voltage-gated calcium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in calcium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of presynaptic cytosolic calcium ion concentration	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of presynaptic cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of synaptic vesicle exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dense core granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neuronal calcium sensor 1

Names: NCS-1; frequenin-like protein; frequenin-like ubiquitous protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_024366.2 → NP_077342.1 neuronal calcium sensor 1

See identical proteins and their annotated locations for NP_077342.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000003

UniProtKB/Swiss-Prot

P62168

UniProtKB/TrEMBL

A6JU20

Related

ENSRNOP00000091251.1, ENSRNOT00000111270.2

Conserved Domains (1) summary

COG5126
Location:20 → 179

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]