BNI1 formin BNI1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BNI1
Official Full Name: formin BNI1
Primary source: SGD:S000005215
Locus tag: YNL271C
See related: AllianceGenome:SGD:S000005215; FungiDB:YNL271C; VEuPathDB:YNL271C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PPF3; SHE5
Summary: Enables profilin binding activity. Involved in several processes, including barbed-end actin filament capping; budding cell apical bud growth; and cytoskeleton organization. Located in several cellular components, including actin filament; cellular bud tip; and incipient cellular bud site. Part of polarisome. Is active in cytosol and mating projection tip. Human ortholog(s) of this gene implicated in autosomal dominant auditory neuropathy 1; autosomal dominant nonsyndromic deafness 1; primary ovarian insufficiency; primary ovarian insufficiency 2A; and sensorineural hearing loss. Orthologous to several human genes including DIAPH2 (diaphanous related formin 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See BNI1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (129522..135383, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We studied the interactions between actin and the FH2 domains of formins Cdc12, Bni1 and mDia1 to understand the factors underlying their different rates of polymerization. All-atom molecular dynamics simulations revealed two factors that influence actin filament elongation and correlate with the rates of elongation
Title: Gating mechanisms during actin filament elongation by formins.
Expression of a hyperactive variant of Bni1 (Bni1-V360D) rescues these defects and partially restores proper spindle positioning in the mutant, suggesting that the failure of dma1 dma2 mutant cells to position the spindle is partly due to faulty formin activity.
Title: Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2.
Myo1 plays a major role in the septin-dependent Mlc1 localization to the division site before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament-dependent Mlc1 localization during cytokinesis.
Title: Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.
F-actin stabilization is regulated by yeast formins Bni1p and Bnr1p
Title: Importance of a Lys113-Glu195 intermonomer ionic bond in F-actin stabilization and regulation by yeast formins Bni1p and Bnr1p.
Data indicate that small forces on the filaments dramatically slowed formin Bni1p-mediated polymerization in the absence of profilin, but resulted in faster polymerization in the presence of profilin.
Title: Tension modulates actin filament polymerization mediated by formin and profilin.
four regions in Bni1p that contribute to its localization to the bud and at the bud neck were difined
Title: Yeast formin Bni1p has multiple localization regions that function in polarized growth and spindle orientation.
A novel regulatory input to Bni1p acting through the Cdc42p effector, Gic2p.
Title: Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p.
data suggest that the R256H actin mutation alters filament conformation resulting in filament instability and misregulation by formin
Title: Thoracic aortic aneurysm (TAAD)-causing mutation in actin affects formin regulation of polymerization.
During polarization, cells change from fast to slow cable dynamics through spatio-temporal regulation of Bni1, Bnr1 and Myo2.
Title: Cortical actin dynamics driven by formins and myosin V.
Yeast expressing the nonlocalized actin nucleating/assembly formin homology (FH) 1-FH2 domains of Bnr1p or Bni1p as the sole formin grow well.
Title: Polarized growth in budding yeast in the absence of a localized formin.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables profilin binding	IDA Inferred from Direct Assay more info	PubMed
enables profilin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in actin filament bundle assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament bundle assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actin filament bundle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in barbed-end actin filament capping	IDA Inferred from Direct Assay more info	PubMed
involved_in budding cell apical bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in budding cell apical bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formin-nucleated actin cable assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in formin-nucleated actin cable assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in formin-nucleated actin cable assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic actomyosin contractile ring assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic actomyosin contractile ring assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in cell division site	IDA Inferred from Direct Assay more info	PubMed
located_in cell division site	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cellular bud neck	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cellular bud tip	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
is_active_in mating projection tip	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic actomyosin contractile ring	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of polarisome	IDA Inferred from Direct Assay more info	PubMed
part_of polarisome	IPI Inferred from Physical Interaction more info	PubMed
located_in prospore membrane	HDA more info	PubMed

General protein information

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Preferred Names: formin BNI1

NP_014128.2

Formin; polarisome component; nucleates the formation of linear actin filaments, involved in budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; spatially focuses Bni1p at mating projection tips, and with Bud6p organizes Bni1p into clusters that nucleate actin assembly during mating projection formation; some functional redundancy with BNR1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

129522..135383 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183109.2 → NP_014128.2 TPA: formin BNI1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014128.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0S3, O13450, P41832

UniProtKB/TrEMBL

B3LPB0, C7GP92, C8ZFW8

Conserved Domains (5) summary

PTZ00121
Location:1739 → 1841

PTZ00121; MAEBL; Provisional

COG1196
Location:703 → 979

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

pfam06367
Location:511 → 707

Drf_FH3; Diaphanous FH3 Domain

pfam02181
Location:1348 → 1741

FH2; Formin Homology 2 Domain

pfam06371
Location:178 → 458

Drf_GBD; Diaphanous GTPase-binding Domain