NAM2 leucine--tRNA ligase NAM2 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: NAM2
Official Full Name: leucine--tRNA ligase NAM2
Primary source: SGD:S000004374
Locus tag: YLR382C
See related: AllianceGenome:SGD:S000004374; FungiDB:YLR382C; VEuPathDB:YLR382C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MSL1
Summary: Enables leucine-tRNA ligase activity and pre-mRNA intronic binding activity. Involved in Group I intron splicing; leucyl-tRNA aminoacylation; and mitochondrial translation. Located in mitochondrion. Used to study Perrault syndrome and mitochondrial metabolism disease. Human ortholog(s) of this gene implicated in Perrault syndrome. Orthologous to human LARS2 (leucyl-tRNA synthetase 2, mitochondrial). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See NAM2 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (882067..884751, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain has been reported.
Title: Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain.
Data show that the activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.
Title: Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.
yeast mitochondria have evolved to tolerate lower levels of fidelity in protein synthesis or have developed alternate mechanisms to enhance discrimination of leucine from non-cognate amino acids that can be misactivated by leucyl-tRNA synthetase
Title: A viable amino acid editing activity in the leucyl-tRNA synthetase CP1-splicing domain is not required in the yeast mitochondria.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA editing activity	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables leucine-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables leucine-tRNA ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables leucine-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables pre-mRNA intronic binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in Group I intron splicing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in aminoacyl-tRNA metabolism involved in translational fidelity	IEA Inferred from Electronic Annotation more info
involved_in leucyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in leucyl-tRNA aminoacylation	IDA Inferred from Direct Assay more info	PubMed
involved_in leucyl-tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: leucine--tRNA ligase NAM2

NP_013486.3

EC 6.1.1.4

Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

882067..884751 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182271.3 → NP_013486.3 TPA: leucine--tRNA ligase NAM2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013486.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZ17, P11325

UniProtKB/TrEMBL

B3RHP7, C7GXI6, C8ZDZ2, N1P047

Conserved Domains (1) summary

TIGR00396
Location:21 → 893

leuS_bact; leucyl-tRNA synthetase, eubacterial and mitochondrial family