SEC4 Rab family GTPase SEC4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SEC4
Official Full Name: Rab family GTPase SEC4
Primary source: SGD:S000001889
Locus tag: YFL005W
See related: AllianceGenome:SGD:S000001889; FungiDB:YFL005W; VEuPathDB:YFL005W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SRO6
Summary: Enables GTP binding activity and GTPase activity. Involved in several processes, including Golgi to plasma membrane transport; ascospore-type prospore assembly; and membrane addition at site of cytokinesis. Located in incipient cellular bud site; mating projection tip; and transport vesicle. Used to study Dyggve-Melchior-Clausen disease and Smith-McCort dysplasia. Orthologous to several human genes including RAB8A (RAB8A, member RAS oncogene family). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SEC4 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (130334..130981)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that mutations in Rab GTPase Sec4p (SEC4) disrupt actin patch assembly and proper endocytic internalization.
Title: Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.
Sec4p phosphorylation facilitates the coordination of membrane-trafficking events during cytokinesis.
Title: Cell cycle-dependent phosphorylation of Sec4p controls membrane deposition during cytokinesis.
Authors describe the identification of a Sec4 binding site on the surface of Sro7 that is contained within a cleft created by the junction of two adjacent beta-propellers that form the core structure of Sro7.
Title: Structural basis for recognition of the Sec4 Rab GTPase by its effector, the Lgl/tomosyn homologue, Sro7.
Results show that Sec4p serine 29 forms a hydrogen bond with the nucleotide and its can form stable associations with Sec2p allowing to elucidate the structure of the Sec4p-Sec2p complex representing an early stage of the exchange reaction.
Title: New insights into the molecular mechanism of the Rab GTPase Sec4p activation.
Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers and highly dependent on both Sro7 and Rab GTPase, Sec4.
Title: In vitro reconstitution of Rab GTPase-dependent vesicle clustering by the yeast lethal giant larvae/tomosyn homolog, Sro7.
Data suggest that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation.
Title: Post-Golgi Sec proteins are required for autophagy in Saccharomyces cerevisiae.
Sec2 is a highly active exchange factor, in contrast to other proteins previously characterized as Rab exchange factors.
Title: Sec2 is a highly efficient exchange factor for the Rab protein Sec4.
The crystal structure of the Sec2p guanine nucleotide exchange factor domain in a complex with the nucleotide-free Sec4p at 2.7 A resolution, is reported.
Title: Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ascospore-type prospore assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in membrane addition at site of cytokinesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in membrane addition at site of cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle fusion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in transport vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in transport vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rab family GTPase SEC4

NP_116650.1

Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane; exocyst association is negatively regulated via phosphorylation by Cdc5p during cytokinesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

130334..130981

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179961.1 → NP_116650.1 TPA: Rab family GTPase SEC4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116650.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTM4, P07560

UniProtKB/TrEMBL

A7A233, B3LUK4, B5VI21, C7GYA2, C8Z7T0, G2WDD5, N1P371

Conserved Domains (1) summary

cd01867
Location:18 → 183

Rab8_Rab10_Rab13_like; Rab GTPase families 8, 10, 13 (Rab8, Rab10, Rab13)