DFM1 Dfm1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DFM1
Official Full Name: Dfm1p
Primary source: SGD:S000002819
Locus tag: YDR411C
See related: AllianceGenome:SGD:S000002819; FungiDB:YDR411C; VEuPathDB:YDR411C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein folding chaperone. Involved in ERAD pathway; endoplasmic reticulum unfolded protein response; and regulation of retrograde protein transport, ER to cytosol. Is active in endoplasmic reticulum. Orthologous to several human genes including DERL1 (derlin 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DFM1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1293369..1294394, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The derlin Dfm1 couples retrotranslocation of a folded protein domain to its proteasomal degradation.
Title: The derlin Dfm1 couples retrotranslocation of a folded protein domain to its proteasomal degradation.
Yeast derlin Dfm1 employs a chaperone-like function to resolve misfolded membrane protein stress.
Title: Yeast derlin Dfm1 employs a chaperone-like function to resolve misfolded membrane protein stress.
Derlin rhomboid pseudoproteases employ substrate engagement and lipid distortion to enable the retrotranslocation of ERAD membrane substrates.
Title: Derlin rhomboid pseudoproteases employ substrate engagement and lipid distortion to enable the retrotranslocation of ERAD membrane substrates.
Results show Ypf1 is an Endoplasmic Reticulum-resident aspartyl intramembrane protease with a presenilin fold, and identified Doa10 and Dfm1 as its cofactors.
Title: The yeast ER-intramembrane protease Ypf1 refines nutrient sensing by regulating transporter abundance.
Dfm1 was part of complexes which contain the ERAD-L ligase Hrd1/Der3 and Der1 as well as the ERAD-C ligase Doa10. Dfm1 forms a complex with the AAA-ATPase Cdc48 in a strain lacking the Cdc48 membrane-recruiting component Ubx2.
Title: Dfm1 forms distinct complexes with Cdc48 and the ER ubiquitin ligases and is required for ERAD.
Dfm1p degradation complex contains the Cdc48p cofactors Ubx1p and Ubx7p
Title: The ER-associated degradation component Der1p and its homolog Dfm1p are contained in complexes with distinct cofactors of the ATPase Cdc48p.
Dfm1p interacts with Cdc48p through its SHP boxes, and so defines a new motif for interaction with this widely-employed AAA-ATPase.
Title: Yeast Derlin Dfm1 interacts with Cdc48 and functions in ER homeostasis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein folding chaperone	IDA Inferred from Direct Assay more info	PubMed
enables signal recognition particle binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in ERAD pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum unfolded protein response	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum unfolded protein response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein folding	IEA Inferred from Electronic Annotation more info
involved_in regulation of retrograde protein transport, ER to cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to stress	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Dfm1p

NP_010699.1

Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; acts as disaggregase that removes misfolded membrane protein aggregates; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1293369..1294394 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180719.1 → NP_010699.1 TPA: Dfm1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010699.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT43, Q12743, Q7LIJ5

UniProtKB/TrEMBL

B3LFP3, C7GY32, C8Z5X4, N1P7E8

Conserved Domains (1) summary

COG5291
Location:9 → 341

COG5291; Predicted membrane protein [Function unknown]