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    TRM82 Trm82p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851743, updated on 9-Dec-2024

    Summary

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    Official Symbol
    TRM82
    Official Full Name
    Trm82p
    Primary source
    SGD:S000002572
    Locus tag
    YDR165W
    See related
    AllianceGenome:SGD:S000002572; FungiDB:YDR165W; VEuPathDB:YDR165W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables enzyme activator activity. Involved in tRNA (guanine-N7)-methylation. Located in cytosol and nucleus. Part of tRNA (m7G46) methyltransferase complex. Used to study Seckel syndrome. Human ortholog(s) of this gene implicated in Galloway-Mowat syndrome and microcephaly, growth deficiency, seizures, and brain malformations. Orthologous to human WDR4 (WD repeat domain 4). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See TRM82 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (784871..786205)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene U2-type spliceosomal complex subunit CWC15 Neighboring gene Sec1p Neighboring gene exocyst subunit SEC5 Neighboring gene Taf10p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Muneyoshi Y, et al. Nucleic Acids Symp Ser (Oxf), 2007. PMID 18029735
    2. Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. Matsumoto K, et al. J Biotechnol, 2008 Feb 29. PMID 18164779
    3. Structure of the yeast tRNA m7G methylation complex. Leulliot N, et al. Structure, 2008 Jan. PMID 18184583
    4. RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex). Matsumoto K, et al. FEBS Lett, 2007 Apr 17. PMID 17382321
    5. tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p. Alexandrov A, et al. RNA, 2005 May. PMID 15811913, Free PMC Article

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. a second role of Trm82p is to stabilize Trm8p in an active conformation
      Title: tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables enzyme activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enzyme activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA (guanine-N7)-methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA (guanine-N7)-methylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA (guanine-N7)-methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA (guanine-N7)-methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of tRNA (m7G46) methyltransferase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of tRNA methyltransferase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    Trm82p
    NP_010449.1
    • Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      784871..786205
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180472.1 → NP_010449.1  TPA: Trm82p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010449.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B3LGB9, B5VG60, D6VSE5, Q03774
      UniProtKB/TrEMBL
      C7GQF4
      Conserved Domains (2) summary
      sd00039
      Location:7 → 46
      7WD40; WD40 repeat [structural motif]
      cl29593
      Location:112 → 278
      WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03774.1

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