RRP8 25S rRNA (adenine645-N1)-methyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RRP8
Official Full Name: 25S rRNA (adenine645-N1)-methyltransferase
Primary source: SGD:S000002490
Locus tag: YDR083W
See related: AllianceGenome:SGD:S000002490; FungiDB:YDR083W; VEuPathDB:YDR083W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables rRNA (adenine) methyltransferase activity. Involved in rRNA methylation and ribosomal large subunit biogenesis. Located in nucleolus. Orthologous to human RRP8 (ribosomal RNA processing 8). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RRP8 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (612073..613251)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The data highlight a dual functionality of Rrp8 in the biogenesis of both small and large ribosomal subunits.
Title: Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables rRNA (adenine) methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables rRNA (adenine) methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables rRNA (adenine-N1-)-methyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in rRNA methylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in rRNA methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in rRNA processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosomal large subunit biogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in ribosomal large subunit biogenesis	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 25S rRNA (adenine645-N1)-methyltransferase

NP_010368.5

EC 2.1.1.287

Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with gar1 mutation; deletion disrupts telomere maintenance by influencing expression of neighboring gene STN1; mammalian homolog nucleomethylin has been linked to nutrient availability signaling pathways and obesity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

612073..613251

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180391.3 → NP_010368.5 TPA: 25S rRNA (adenine645-N1)-methyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010368.5

Status: REVIEWED

UniProtKB/Swiss-Prot

P38961, Q7LHG2

UniProtKB/TrEMBL

C7GVQ3, C8Z501, N1P850

Conserved Domains (1) summary

cl17173
Location:117 → 392

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...