PIS1 CDP-diacylglycerol--inositol 3-phosphatidyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PIS1
Official Full Name: CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Primary source: SGD:S000006317
Locus tag: YPR113W
See related: AllianceGenome:SGD:S000006317; FungiDB:YPR113W; VEuPathDB:YPR113W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity. Involved in phosphatidylinositol biosynthetic process. Located in Golgi apparatus and mitochondrial outer membrane. Used to study cancer. Orthologous to human CDIPT (CDP-diacylglycerol--inositol 3-phosphatidyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PIS1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (752257..752919)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cytosolically made inositol and CDP-diacylglycerol can access the active site of the yeast PI synthase Pis1 from the cytosolic side and that Pis1 structure is strongly affected by mild detergents.
Title: The active site of yeast phosphatidylinositol synthase Pis1 is facing the cytosol.
Ume6p was found to be a positive regulator of PIS1 gene expression.
Title: Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p.
multiple upstream AUG codons are present in at least some PIS1 transcripts and negatively impact PIS1 expression
Title: Expression of the Saccharomyces cerevisiae PIS1 gene is modulated by multiple ATGs in the promoter.
This study employed a genomic screen, database mining, and more traditional promoter analysis to identify genes that affect PIS1 expression.
Title: Genomic analysis of PIS1 gene expression.
PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae is regulated by zinc
Title: Regulation of the PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae by zinc.
Enhanced phosphatidylinositol synthesis may account for PIS1 suppression of rsp5 defects.
Title: Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphotransferase activity, for other substituted phosphate groups	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in lipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylinositol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: CDP-diacylglycerol--inositol 3-phosphatidyltransferase

NP_015438.1

EC 2.7.8.11

Phosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

752257..752919

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184210.1 → NP_015438.1 TPA: CDP-diacylglycerol--inositol 3-phosphatidyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015438.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4B2, P06197

UniProtKB/TrEMBL

A6ZWZ4, B3LK77, B5VTQ3, C7GXS5, C8ZJD5, G2WPT0, N1NVL1

Conserved Domains (1) summary

COG0558
Location:6 → 175

PgsA; Phosphatidylglycerophosphate synthase [Lipid transport and metabolism]