RNH201 ribonuclease H2 catalytic subunit RNH201 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RNH201
Official Full Name: ribonuclease H2 catalytic subunit RNH201
Primary source: SGD:S000005016
Locus tag: YNL072W
See related: AllianceGenome:SGD:S000005016; FungiDB:YNL072W; VEuPathDB:YNL072W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RNH35
Summary: Enables RNA-DNA hybrid ribonuclease activity. Involved in DNA replication, removal of RNA primer. Located in nucleus. Part of ribonuclease H2 complex. Used to study Aicardi-Goutieres syndrome. Human ortholog(s) of this gene implicated in Aicardi-Goutieres syndrome. Orthologous to human RNASEH2A (ribonuclease H2 subunit A). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RNH201 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (490317..491240)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ribodysgenesis: sudden genome instability in the yeast Saccharomyces cerevisiae arising from RNase H2 cleavage at genomic-embedded ribonucleotides.
Title: Ribodysgenesis: sudden genome instability in the yeast Saccharomyces cerevisiae arising from RNase H2 cleavage at genomic-embedded ribonucleotides.
Aicardi-Goutieres syndrome-associated mutations in rnh201 have strong negative impact on genome stability in yeast.
Title: Genome instability consequences of RNase H2 Aicardi-Goutières syndrome alleles.
Both R-loop removal and RER activities of RNase H2 contribute substantially to chromosome stability, and that their relative contributions may be variable across different regions of the genome. In this scenario, a prominent contribution of R-loop removal may be expected at highly transcribed regions, whereas RER may play a greater role at hotspots of ribonucleotide incorporation.
Title: Both R-loop removal and ribonucleotide excision repair activities of RNase H2 contribute substantially to chromosome stability.
An important role for RNase H2 in maintaining genome integrity by removing single ribonucleotides incorporated during DNA replication is shown.
Title: The role of RNase H2 in processing ribonucleotides incorporated during DNA replication.
high-resolution mapping of mitotic recombination events throughout the yeast genome in diploid strains of Saccharomyces cerevisiae lacking RNase H1 (rnh1Delta), RNase H2 (rnh201Delta), or both RNase H1 and RNase H2 (rnh1Delta rnh201Delta)
Title: Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity.
Deleting RNH201 elicits a strong cellular stress response in S. cerevisiae.
Title: Transcriptional responses to loss of RNase H2 in Saccharomyces cerevisiae.
study presents a complete biochemical reconstitution of the ribonucleotide excision repair (RER) pathway; RER is most efficient when the ribonucleotide is incised by RNase H2
Title: RNase H2-initiated ribonucleotide excision repair.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA-DNA hybrid ribonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA-DNA hybrid ribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA-DNA hybrid ribonuclease activity	IEA Inferred from Electronic Annotation more info
enables RNA-DNA hybrid ribonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA replication, removal of RNA primer	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication, removal of RNA primer	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA replication, removal of RNA primer	IGI Inferred from Genetic Interaction more info	PubMed
involved_in RNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonuclease H2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonuclease H2 complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ribonuclease H2 catalytic subunit RNH201

NP_014327.1

EC 3.1.26.4

Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

490317..491240

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182910.1 → NP_014327.1 TPA: ribonuclease H2 catalytic subunit RNH201 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014327.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1A7, P53942

UniProtKB/TrEMBL

B3LNT1, B5VQX3, C7GIL4, N1P4H1

Conserved Domains (1) summary

cd07181
Location:36 → 260

RNase_HII_eukaryota_like; Eukaryotic RNase HII