MGS1 ssDNA-dependent ATPase MGS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MGS1
Official Full Name: ssDNA-dependent ATPase MGS1
Primary source: SGD:S000005162
Locus tag: YNL218W
See related: AllianceGenome:SGD:S000005162; FungiDB:YNL218W; VEuPathDB:YNL218W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables G-quadruplex DNA binding activity; enzyme activator activity; and single-stranded DNA helicase activity. Involved in DNA replication, Okazaki fragment processing; chromosome organization; and regulation of DNA repair. Located in cytoplasm and nucleus. Orthologous to human WRNIP1 (WRN helicase interacting protein 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MGS1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (238238..240001)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mgs1 function at G-quadruplex structures during DNA replication.
Title: Mgs1 function at G-quadruplex structures during DNA replication.
Prevention of unwanted recombination at damaged replication forks.
Title: Prevention of unwanted recombination at damaged replication forks.
Mgs1 protein supports genome stability via recognition of G-quadruplex DNA structures.
Title: Mgs1 protein supports genome stability via recognition of G-quadruplex DNA structures.
Mgs1 is a negative regulator of DNA polymerase zeta in ultraviolet mutagenesis.
Title: A novel variant of DNA polymerase ζ, Rev3ΔC, highlights differential regulation of Pol32 as a subunit of polymerase δ versus ζ in Saccharomyces cerevisiae.
By means of preferential physical interactions with the ubiquitylated forms of the replicative sliding clamp, proliferating cell nuclear antigen, the UBZ domain facilitates recruitment of Mgs1 to sites of replication stress.
Title: The genome maintenance factor Mgs1 is targeted to sites of replication stress by ubiquitylated PCNA.
Study suggests that Rad18/Rad5/Mms2 and Mgs1 are required to promote replication when forks are destabilized or stalled due to defects in Pol delta.
Title: Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31.
Mgs1 physically associates with PCNA and that Mgs1 helps suppress the RAD6 DNA damage tolerance pathway in the absence of exogenous DNA damage.
Title: Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance.
Mgs1 is involved in Okazaki fragment processing via modulation of Fen1 activity.
Title: In vivo and in vitro studies of Mgs1 suggest a link between genome instability and Okazaki fragment processing.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables enzyme activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication, Okazaki fragment processing	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA synthesis involved in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ssDNA-dependent ATPase MGS1

NP_014181.1

Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

238238..240001

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183056.1 → NP_014181.1 TPA: ssDNA-dependent ATPase MGS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014181.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0X2, P40151

UniProtKB/TrEMBL

A6ZRM2, B3LP62, B5VQJ3, C7GPD9, C8ZG15, G2WLP5, N1NWE3

Conserved Domains (3) summary

smart00734
Location:11 → 33

ZnF_Rad18; Rad18-like CCHC zinc finger

COG2256
Location:134 → 576

RarA; Replication-associated recombination protein RarA (DNA-dependent ATPase) [Replication, recombination and repair]

COG5432
Location:2 → 147

RAD18; RING-finger-containing E3 ubiquitin ligase [Signal transduction mechanisms]