CDC5 polo kinase CDC5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CDC5
Official Full Name: polo kinase CDC5
Primary source: SGD:S000004603
Locus tag: YMR001C
See related: AllianceGenome:SGD:S000004603; FungiDB:YMR001C; VEuPathDB:YMR001C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MSD2; PKX2
Summary: Enables centromeric DNA binding activity; phosphoprotein binding activity; and protein serine/threonine kinase activity. Involved in several processes, including G2/M transition of mitotic cell cycle; nuclear division; and regulation of protein localization. Acts upstream of or within regulation of protein localization to nucleolus. Located in cellular bud neck; microtubule cytoskeleton; and nucleus. Human ortholog(s) of this gene implicated in urinary bladder cancer. Orthologous to several human genes including PLK1 (polo like kinase 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CDC5 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (269019..271136, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Polo kinase Cdc5 is regulated at multiple levels in the adaptation response to telomere dysfunction.
Title: The Polo kinase Cdc5 is regulated at multiple levels in the adaptation response to telomere dysfunction.
The N-Terminal Region of the Polo Kinase Cdc5 Is Required for Downregulation of the Meiotic Recombination Checkpoint.
Title: The N-Terminal Region of the Polo Kinase Cdc5 Is Required for Downregulation of the Meiotic Recombination Checkpoint.
Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.
Title: Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.
we confirmed that this release indeed accompanies the dissociation of acetylated Smc3 as well as Rec8 from meiotic chromosomes during late prophase-I. This release requires, in addition to PLK, the cohesin regulator, Wapl (Rad61/Wpl1 in yeast), and Dbf4-dependent Cdc7 kinase (DDK)
Title: Meiosis-specific prophase-like pathway controls cleavage-independent release of cohesin by Wapl phosphorylation.
Cdc5 interacts with cohesin in vivo, and cohesin is required for association of Cdc5 at centromeric chromatin. Cohesin removal from centromeric chromatin requires Cdc5 but removal at distal chromosomal arm sites does not. Results define a novel role for Cdc5 in regulating removal of centromeric cohesins and faithful chromosome segregation.
Title: Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis.
budding yeast Plk1, Cdc5, is pre-deposited onto cohesin engaged in cohesion on chromosome arms in G2/M phase cells. The Cdc5-cohesin association is mediated by direct interaction between the polo-box domain of Cdc5 and Scc1 phosphorylated at multiple sites in its middle region.
Title: Physical Association of Saccharomyces cerevisiae Polo-like Kinase Cdc5 with Chromosomal Cohesin Facilitates DNA Damage Response.
Polo kinase Cdc5 mediates centrosome-dependent bypass of the DNA damage checkpoint.
Title: Centrosome-Dependent Bypass of the DNA Damage Checkpoint by the Polo Kinase Cdc5.
Small N-terminal regions of both Cdc20 (RxxL-type D box) and Cdc5 (KEN motif) were identified as transferable degradation domains (degrons) that mediated efficient targeting by APC/C-Cdh1 (anaphase-promoting complex/cyclosome-Cdh1).
Title: Insights into the cellular mechanism of the yeast ubiquitin ligase APC/C-Cdh1 from the analysis of in vivo degrons.
Cin8 mutants lacking critical CDK phosphorylation sites suppress the requirement for Cdc14 and Cdc5 in anaphase spindle elongation.
Title: FEAR-mediated activation of Cdc14 is the limiting step for spindle elongation and anaphase progression.
Misregulated expression of Cdc5 causes increased mitotic crossover frequency.
Title: Cell-cycle kinases coordinate the resolution of recombination intermediates with chromosome segregation.

Submit: New GeneRIF Correction See all GeneRIFs (33)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	IPI Inferred from Physical Interaction more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in exit from mitosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of protein localization to nucleolus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of protein localization to nucleolus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Rho protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic spindle pole body separation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of mitotic spindle pole body separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to cell division site involved in mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nucleus organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization to mitotic spindle pole body	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of protein localization to nucleolus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptonemal complex disassembly	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
is_active_in cellular bud neck	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle pole body	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole body	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: polo kinase CDC5

NP_013714.1

EC 2.7.11.21

Polo-like kinase required for mitotic exit; regulates mitotic spindle assembly, nuclear shape, and protein localization to the nucleolus and SPBs; controls targeting and activation of Rho1p at the division site via Rho1p-GEFs; role in adaptation to DNA damage promotes genome instability during replicative senescence; regulates meiotic commitment, spindle assembly, chiasmata formation, recombination intermediate resolution and SC disassembly; human homologs PLK1 and PLK3 complement cdc5 mutants

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

269019..271136 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182497.1 → NP_013714.1 TPA: polo kinase CDC5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013714.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZH5, P32562

UniProtKB/TrEMBL

A6ZM62, B3LLQ1, C7GLB8, G2WK52, N1NX49

Conserved Domains (3) summary

cd13117
Location:613 → 698

POLO_box_2; Second polo-box domain (PBD) of polo-like kinases Plk1, Plk2, Plk3, and Plk5

cd13118
Location:510 → 601

POLO_box_1; First polo-box domain (PBD) of polo-like kinases Plk1, Plk2, Plk3, and Plk5

cd14099
Location:80 → 337

STKc_PLK; Catalytic domain of the Serine/Threonine Kinases, Polo-like kinases