SPP382 mRNA splicing protein SPP382 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPP382
Official Full Name: mRNA splicing protein SPP382
Primary source: SGD:S000004416
Locus tag: YLR424W
See related: AllianceGenome:SGD:S000004416; FungiDB:YLR424W; VEuPathDB:YLR424W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CCF8; NTR1
Summary: Contributes to RNA helicase activity. Involved in generation of catalytic spliceosome for first transesterification step and spliceosomal complex disassembly. Located in mitochondrion and nucleus. Part of U2-type post-mRNA release spliceosomal complex. Orthologous to human TFIP11 (tuftelin interacting protein 11). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SPP382 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (973395..975521)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our data suggest that Cwc23 is important for maintaining the levels of Ntr1 and Ntr2, and that it also plays a regulatory role in targeting spliceosome intermediates for disassembly.
Title: Cwc23 is a component of the NTR complex and functions to stabilize Ntr1 and facilitate disassembly of spliceosome intermediates.
Ntr1 and Ntr2 may act as 'doorkeepers' and both cofactors may inspect the RNP structure of spliceosomal complexes thereby targeting suboptimal spliceosomes for Prp43-mediated disassembly.
Title: Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2.
Prp43 associates with Ntr1 and Ntr2 to form the NTR complex which mediates the disassembly of spliceosome intermediates.
Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.
Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.
Title: Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.
These results demonstrate that dynamic interactions of NTR with U5, through the interaction of Ntr2 with Brr2, and interactions of Ntr1 and Prp43 govern the recruitment of Prp43 to the spliceosome to mediate spliceosome disassembly.
Title: Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly.
Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome.
Title: Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome.
Ntr1-Ntr2-Prp43 complex catalyze disassembly of the spliceosome in an ATP-dependent manner
Title: Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.
Ntr1 promotes release of excised introns from splicing complexes by acting as a spliceosome receptor or RNA-targeting factor for Prp43, possibly assisted by the Ntr2 protein.
Title: Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.
Ntr1p interacts with Lif1p in a two-hybrid screen.
Title: Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in generation of catalytic spliceosome for first transesterification step	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in spliceosomal complex disassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in spliceosomal complex disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in spliceosomal complex disassembly	IEA Inferred from Electronic Annotation more info
involved_in spliceosomal complex disassembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spliceosomal complex disassembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of U2-type post-mRNA release spliceosomal complex	IBA Inferred from Biological aspect of Ancestor more info
part_of U2-type post-mRNA release spliceosomal complex	IDA Inferred from Direct Assay more info	PubMed
part_of U2-type post-mRNA release spliceosomal complex	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mRNA splicing protein SPP382

NP_013528.1

Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

973395..975521

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182312.1 → NP_013528.1 TPA: mRNA splicing protein SPP382 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013528.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZ60, Q06411

UniProtKB/TrEMBL

B3RHT4, B5VNV9, N1P7F0

Conserved Domains (1) summary

pfam01585
Location:61 → 104

G-patch; G-patch domain