PFK2 6-phosphofructokinase subunit beta [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PFK2
Official Full Name: 6-phosphofructokinase subunit beta
Primary source: SGD:S000004818
Locus tag: YMR205C
See related: AllianceGenome:SGD:S000004818; FungiDB:YMR205C; VEuPathDB:YMR205C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA binding activity. Contributes to 6-phosphofructokinase activity. Involved in glycolytic process; vacuolar acidification; and vacuolar proton-transporting V-type ATPase complex assembly. Located in mitochondrion. Part of 6-phosphofructokinase complex. Used to study glycogen storage disease. Human ortholog(s) of this gene implicated in glycogen storage disease VII. Orthologous to human PFKL (phosphofructokinase, liver type) and PFKM (phosphofructokinase, muscle). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PFK2 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (671887..674766, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Binding of disassembled V-ATPase (V1 domain) to its assembly factor RAVE (subunit Rav1p) was 5-fold enhanced, indicating that Pfk2p is necessary for V-ATPase regulation by glucose.
Title: Yeast phosphofructokinase-1 subunit Pfk2p is necessary for pH homeostasis and glucose-dependent vacuolar ATPase reassembly.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 6-phosphofructokinase activity	IEA Inferred from Electronic Annotation more info
contributes_to 6-phosphofructokinase activity	IGI Inferred from Genetic Interaction more info	PubMed
contributes_to 6-phosphofructokinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuolar acidification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuolar proton-transporting V-type ATPase complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of 6-phosphofructokinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructokinase complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 6-phosphofructokinase subunit beta

NP_013932.1

EC 2.7.1.11

Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

671887..674766 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182712.1 → NP_013932.1 TPA: 6-phosphofructokinase subunit beta [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013932.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W030, P16862

UniProtKB/TrEMBL

B3LM93, N1P0I3

Conserved Domains (3) summary

cd06587
Location:17 → 140

Glo_EDI_BRP_like; This domain superfamily is found in a variety of structurally related metalloproteins, including the type I extradiol dioxygenases, glyoxalase I and a group of antibiotic resistance proteins

TIGR02478
Location:197 → 957

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

cl00204
Location:196 → 947

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...