U2af2 U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: U2af2provided by MGI
Official Full Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2provided by MGI
Primary source: MGI:MGI:98886
See related: Ensembl:ENSMUSG00000030435 AllianceGenome:MGI:98886
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 65kDa
Summary: Enables C2H2 zinc finger domain binding activity. Involved in RNA splicing. Predicted to be part of Prp19 complex; U2-type spliceosomal complex; and U2AF complex. Predicted to be active in nuclear speck. Is expressed in several structures, including central nervous system; gut; liver lobe; lower urinary tract; and nose. Human ortholog(s) of this gene implicated in developmental delay, dysmorphic facies, and brain anomalies. Orthologous to human U2AF2 (U2 small nuclear RNA auxiliary factor 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 88.3), CNS E14 (RPKM 83.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 A1; 7 2.93 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (5063884..5082937)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (5060885..5079945)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2.
Title: Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2.
Perturbation of U2AF65/NXF1-mediated RNA nuclear export enhances RNA toxicity in polyQ diseases.
Title: Perturbation of U2AF65/NXF1-mediated RNA nuclear export enhances RNA toxicity in polyQ diseases.
dephosphorylation of pS776-ATXN1 by PP2A regulates the interaction of ATXN1 with the splicing factors RBM17 and U2AF65
Title: 14-3-3 Binding to ataxin-1(ATXN1) regulates its dephosphorylation at Ser-776 and transport to the nucleus.
Using a yeast two-hybrid system, we found fourteen U2AF(65)-interacting proteins. The validity of the screen was confirmed by identification of five known U2AF(65)-interacting proteins, including its heterodimeric partner, U2AF(35).
Title: Interactome for auxiliary splicing factor U2AF(65) suggests diverse roles.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

U2af2 (e-PCR), detects polymorphism
- UniSTS:479300

RH125439 (e-PCR)
- UniSTS:162260

U2AF2_7999 (e-PCR)
- UniSTS:467966

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables C2H2 zinc finger domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables molecular function inhibitor activity	ISO Inferred from Sequence Orthology more info
enables poly-pyrimidine tract binding	IBA Inferred from Biological aspect of Ancestor more info
enables pre-mRNA 3'-splice site binding	IBA Inferred from Biological aspect of Ancestor more info
enables pre-mRNA 3'-splice site binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA splicing	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA splicing, via spliceosome	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of mRNA splicing, via spliceosome	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mRNA splicing, via spliceosome	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of RNA splicing	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of RNA splicing	ISS Inferred from Sequence or Structural Similarity more info
involved_in spliceosomal complex assembly	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Prp19 complex	ISO Inferred from Sequence Orthology more info
part_of Prp19 complex	ISS Inferred from Sequence or Structural Similarity more info
part_of U2-type prespliceosome	IBA Inferred from Biological aspect of Ancestor more info
part_of U2-type prespliceosome	IEA Inferred from Electronic Annotation more info
part_of U2AF complex	IBA Inferred from Biological aspect of Ancestor more info
part_of U2AF complex	ISO Inferred from Sequence Orthology more info
part_of commitment complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nuclear speck	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info	PubMed
located_in nuclear speck	TAS Traceable Author Statement more info	PubMed
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
part_of spliceosomal complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: splicing factor U2AF 65 kDa subunit

Names: U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), 65 kDa; U2 snRNP auxiliary factor large subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001205231.2 → NP_001192160.1 splicing factor U2AF 65 kDa subunit isoform 1

See identical proteins and their annotated locations for NP_001192160.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC162893

Consensus CDS

CCDS57476.1

UniProtKB/Swiss-Prot

P26369

UniProtKB/TrEMBL

Q505Q1

Related

ENSMUSP00000147013.2, ENSMUST00000209099.2

Conserved Domains (1) summary

TIGR01642
Location:63 → 474

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor
NM_001403145.1 → NP_001390074.1 splicing factor U2AF 65 kDa subunit isoform 3

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q505Q1
NM_001403146.1 → NP_001390075.1 splicing factor U2AF 65 kDa subunit isoform 4

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q505Q1
NM_001403147.1 → NP_001390076.1 splicing factor U2AF 65 kDa subunit isoform 5

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q505Q1
NM_001403148.1 → NP_001390077.1 splicing factor U2AF 65 kDa subunit isoform 6

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q505Q1
NM_001403149.1 → NP_001390078.1 splicing factor U2AF 65 kDa subunit isoform 6

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q505Q1
NM_001403150.1 → NP_001390079.1 splicing factor U2AF 65 kDa subunit isoform 7

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q922I0
NM_001403151.1 → NP_001390080.1 splicing factor U2AF 65 kDa subunit isoform 7

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q922I0
NM_001403153.1 → NP_001390082.1 splicing factor U2AF 65 kDa subunit isoform 7

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q922I0
NM_001403154.1 → NP_001390083.1 splicing factor U2AF 65 kDa subunit isoform 8

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q3KQM4, Q922I0
NM_001403155.1 → NP_001390084.1 splicing factor U2AF 65 kDa subunit isoform 8

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q3KQM4, Q922I0
NM_001403156.1 → NP_001390085.1 splicing factor U2AF 65 kDa subunit isoform 8

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q3KQM4, Q922I0
NM_001403158.1 → NP_001390087.1 splicing factor U2AF 65 kDa subunit isoform 8

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q3KQM4, Q922I0
NM_001403159.1 → NP_001390088.1 splicing factor U2AF 65 kDa subunit isoform 8

Status: VALIDATED

Source sequence(s)

AC162893

UniProtKB/TrEMBL

Q3KQM4, Q922I0
NM_133671.4 → NP_598432.2 splicing factor U2AF 65 kDa subunit isoform 2

See identical proteins and their annotated locations for NP_598432.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AC162893

Consensus CDS

CCDS85208.1

UniProtKB/TrEMBL

Q505Q1, Q80XR5

Related

ENSMUSP00000005041.9, ENSMUST00000005041.15

Conserved Domains (1) summary

TIGR01642
Location:63 → 470

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

5063884..5082937

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030242366.1 → XP_030098226.1 splicing factor U2AF 65 kDa subunit isoform X6

UniProtKB/TrEMBL

Q922I0

Conserved Domains (1) summary

TIGR01642
Location:1 → 310

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor
XM_030242364.1 → XP_030098224.1 splicing factor U2AF 65 kDa subunit isoform X1

UniProtKB/TrEMBL

Q505Q1, Q80XR5

Conserved Domains (1) summary

TIGR01642
Location:63 → 470

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor
XM_036152913.1 → XP_036008806.1 splicing factor U2AF 65 kDa subunit isoform X4

UniProtKB/TrEMBL

Q505Q1

Conserved Domains (1) summary

TIGR01642
Location:1 → 370

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor