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    MED30 mediator complex subunit 30 [ Homo sapiens (human) ]

    Gene ID: 90390, updated on 10-Dec-2024

    Summary

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    Official Symbol
    MED30provided by HGNC
    Official Full Name
    mediator complex subunit 30provided by HGNC
    Primary source
    HGNC:HGNC:23032
    See related
    Ensembl:ENSG00000164758 MIM:610237; AllianceGenome:HGNC:23032
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MED30S; THRAP6; TRAP25
    Summary
    The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]
    Expression
    Ubiquitous expression in bone marrow (RPKM 9.2), lymph node (RPKM 5.0) and 24 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See MED30 in Genome Data Viewer
    Location:
    8q24.11
    Exon count:
    5
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 8 NC_000008.11 (117520713..117540262)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 8 NC_060932.1 (118648865..118668421)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 8 NC_000008.10 (118532952..118552501)

    Chromosome 8 - NC_000008.11Genomic Context describing neighboring genes Neighboring gene solute carrier family 30 member 8 Neighboring gene uncharacterized LOC105375716 Neighboring gene RNA, 7SL, cytoplasmic 826, pseudogene Neighboring gene uncharacterized LOC105375717 Neighboring gene NANOG hESC enhancer GRCh37_chr8:118369992-118370493 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr8:118400318-118401517 Neighboring gene ribosomal protein S10 pseudogene 16 Neighboring gene uncharacterized LOC105375721 Neighboring gene uncharacterized LOC107986970

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Mediator subunits: gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells. Rienzo M, et al. Biochim Biophys Acta, 2010 Jul. PMID 20493979
    2. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s. Baek HJ, et al. Mol Cell Biol, 2002 Apr. PMID 11909976, Free PMC Article
    3. The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells. Syring I, et al. Ann Diagn Pathol, 2018 Jun. PMID 29661722
    4. The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer. Syring I, et al. Anticancer Res, 2017 Dec. PMID 29187445
    5. HIF1α and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance. Shukla A, et al. Cell Mol Neurobiol, 2021 Oct. PMID 32705436

    See all (54) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. HIF1alpha and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance.
      Title: HIF1α and p53 Regulated MED30, a Mediator Complex Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and Temozolomide Resistance.
    2. MED30 seems to be involved in the progression of the renal cell carcinoma (RCC).
      Title: The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.
    3. Knockdown of MED30 led to reduction of the tumour parameters proliferation, migration, and invasion in the bladder cancer (BCa) cell lines.
      Title: The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
    4. analysis of a novel transcript of MED30, termed MED30 short (MED30s) generated by alternative splicing
      Title: Mediator subunits: gene expression pattern, a novel transcript identification and nuclear localization in human endothelial progenitor cells.
    5. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.
      Title: Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    EBI GWAS Catalog

    Description
    Two new susceptibility loci for Kawasaki disease identified through genome-wide association analysis.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of mediator complex subunit 30 (MED30) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat SiRNA knockdown of d MED30 shows a modest inhibition in HIV-1 Tat-mediated transcription levels as compared to control PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC9890

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables nuclear receptor coactivator activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables nuclear thyroid hormone receptor binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nuclear vitamin D receptor binding NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in somatic stem cell population maintenance IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of core mediator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ubiquitin ligase complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 30
    Names
    TRAP/Mediator complex component TRAP25
    putative mediator of RNA polymerase II transcription subunit 30
    thyroid hormone receptor-associated protein 6
    thyroid hormone receptor-associated protein complex 25 kDa component

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001282986.2 → NP_001269915.1  mediator of RNA polymerase II transcription subunit 30 isoform 2

      See identical proteins and their annotated locations for NP_001269915.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoofrm 1.
      Source sequence(s)
      AA812233, AC024329, AC087361, FM179284
      Consensus CDS
      CCDS64959.1
      UniProtKB/Swiss-Prot
      Q96HR3
      Related
      ENSP00000431051.1, ENST00000522839.1
      Conserved Domains (1) summary
      pfam11315
      Location:29 → 141
      Med30; Mediator complex subunit 30

    2. NM_001363182.2 → NP_001350111.1  mediator of RNA polymerase II transcription subunit 30 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC024329, AC087361, AY083305
      Conserved Domains (1) summary
      pfam11315
      Location:29 → 147
      Med30; Mediator complex subunit 30

    3. NM_080651.4 → NP_542382.1  mediator of RNA polymerase II transcription subunit 30 isoform 1

      See identical proteins and their annotated locations for NP_542382.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest isoform (1).
      Source sequence(s)
      AA812233, AC024329, AC087361, AY083305
      Consensus CDS
      CCDS6323.1
      UniProtKB/Swiss-Prot
      C6GKU9, Q96HR3
      Related
      ENSP00000297347.3, ENST00000297347.7
      Conserved Domains (1) summary
      pfam11315
      Location:29 → 176
      Med30; Mediator complex subunit 30

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000008.11 Reference GRCh38.p14 Primary Assembly

      Range
      117520713..117540262
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060932.1 Alternate T2T-CHM13v2.0

      Range
      118648865..118668421
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96HR3.1 GenPept UniProtKB/Swiss-Prot:Q96HR3

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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