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    LDB16 Ldb16p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850351, updated on 9-Dec-2024

    Summary

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    Official Symbol
    LDB16
    Official Full Name
    Ldb16p
    Primary source
    SGD:S000000511
    Locus tag
    YCL005W
    See related
    AllianceGenome:SGD:S000000511; FungiDB:YCL005W; VEuPathDB:YCL005W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Involved in lipid droplet organization and negative regulation of sphingolipid biosynthetic process. Located in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See LDB16 in Genome Data Viewer
    Location:
    chromosome: III
    Exon count:
    1
    Sequence:
    Chromosome: III; NC_001135.5 (108021..108791)

    Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene H(+)-transporting V0 sector ATPase subunit e Neighboring gene ncRNA Neighboring gene CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001135.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters. Renne MF, et al. J Cell Biol, 2022 Sep 5. PMID 35938957, Free PMC Article
    2. Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex. Klug YA, et al. Nat Commun, 2021 Oct 8. PMID 34625558, Free PMC Article
    3. Seipin negatively regulates sphingolipid production at the ER-LD contact site. Su WC, et al. J Cell Biol, 2019 Nov 4. PMID 31594806, Free PMC Article
    4. A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides. Corbacho I, et al. Fungal Genet Biol, 2005 Sep. PMID 15993632
    5. The seipin complex Fld1/Ldb16 stabilizes ER-lipid droplet contact sites. Grippa A, et al. J Cell Biol, 2015 Nov 23. PMID 26572621, Free PMC Article

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters.
      Title: Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters.
    2. Proper droplet formation requires the collaboration of Sei1p and Ldb16.
      Title: Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1p(ΔNterm) only in combination with Ldb16p.
    3. The Fld1/Ldb16 complex facilitates the establishment of lipid droplet (LD) identity by acting as a diffusion barrier at the endoplasmic reticulum-LD contact sites.
      Title: The seipin complex Fld1/Ldb16 stabilizes ER-lipid droplet contact sites.
    4. Fld1(seipin)-Ldb16 complex affects phosphatidic acid homeostasis at an lipid droplets-forming subdomain of the nuclear envelope.
      Title: Seipin is involved in the regulation of phosphatidic acid metabolism at a subdomain of the nuclear envelope in yeast.
    5. The stability of Ldb16 requires Fld1, as Ldb16 is subjected to ER-associated degradation (ERAD) in the absence of Fld1 but is stabilized when Fld1 is present.
      Title: Control of lipid droplet size in budding yeast requires the collaboration between Fld1 and Ldb16.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in lipid droplet organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of sphingolipid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in lipid droplet HDA PubMed 
    located_in lipid droplet IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion HDA PubMed 

    General protein information

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    Preferred Names
    Ldb16p
    NP_009922.2
    • Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, stabilizing contact sites; ensures that LDs bud from the ER towards the cytosolic side of the membrane; null mutants have decreased net negative cell surface charge and localized accumulation of phosphatidic acid (PA) marker proteins; GFP-fusion protein expression is induced in response to MMS; null mutant can be complemented by the human seipin, BSCL2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001135.5 Reference assembly

      Range
      108021..108791
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178654.1 → NP_009922.2  TPA: Ldb16p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009922.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VR06, P25587, Q8NIM5
      UniProtKB/TrEMBL
      A6ZTH8, B3LU69, B5VEU6, C7GQL1, C8Z480, G2WA14, N1P994
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25587.2

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