pam-1 Puromycin-sensitive aminopeptidase [Caenorhabditis elegans] - Gene

Summary

Go to the top of the page Help

Official Symbol: pam-1
Official Full Name: Puromycin-sensitive aminopeptidase
Primary source: WormBase:WBGene00003914
Locus tag: CELE_F49E8.3
See related: AllianceGenome:WB:WBGene00003914
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables metalloaminopeptidase activity. Involved in several processes, including exit from meiosis; first cell cycle pseudocleavage; and maintenance of centrosome location. Located in condensed chromosome; cytoplasm; and mitotic spindle pole. Is expressed in amphid process; intestine; male-specific anatomical entity; nerve ring; and tail neurons. Orthologous to several human genes including NPEPPS (aminopeptidase puromycin sensitive). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See pam-1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 8

Sequence:: Chromosome: IV; NC_003282.8 (7543984..7550804, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interactions between the WEE-1.3 kinase and the PAM-1 aminopeptidase in oocyte maturation and the early C. elegans embryo.
Title: Interactions between the WEE-1.3 kinase and the PAM-1 aminopeptidase in oocyte maturation and the early C. elegans embryo.
Data show that the puromycin-sensitive aminopeptidase PAM-1 is required for meiotic exit and anteroposterior polarity in the one-cell Caenorhabditis elegans embryo.
Title: The puromycin-sensitive aminopeptidase PAM-1 is required for meiotic exit and anteroposterior polarity in the one-cell Caenorhabditis elegans embryo.
clusters phylogenetically with the puromycin-sensitive aminopeptidase, despite attenuated sensitivity toward puromycin; functions in embryo development and reproduction of the nematode
Title: The Caenorhabditis elegans orthologue of mammalian puromycin-sensitive aminopeptidase has roles in embryogenesis and reproduction.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

F49E8.4 (e-PCR)
- UniSTS:320553

F49E8.2 (e-PCR)
- UniSTS:311698

F49E8.3 (e-PCR)
- UniSTS:311699

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables aminopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloaminopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloaminopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptide binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in anterior/posterior axis specification, embryo	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exit from meiosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in first cell cycle pseudocleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of centrosome location	IGI Inferred from Genetic Interaction more info	PubMed
involved_in maintenance of centrosome location	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in oogenesis	IEA Inferred from Electronic Annotation more info
involved_in peptide catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of oocyte maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of egg-laying behavior	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of meiotic cell cycle process involved in oocyte maturation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of meiotic cell cycle process involved in oocyte maturation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle pole	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Puromycin-sensitive aminopeptidase

NP_001023210.1

Confirmed by transcript evidence

NP_001379916.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

7543984..7550804 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001392330.1 → NP_001379916.1 Puromycin-sensitive aminopeptidase [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (2) summary

cd09601
Location:23 → 460

M1_APN-Q_like; Peptidase M1 aminopeptidase N catalytic domain family which includes aminopeptidase N (APN), aminopeptidase Q (APQ), tricorn interacting factor F3, and endoplasmic reticulum aminopeptidase 1 (ERAP1)

pfam11838
Location:538 → 863

ERAP1_C; ERAP1-like C-terminal domain
NM_001028039.3 → NP_001023210.1 Puromycin-sensitive aminopeptidase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023210.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q20627, Q4TT88

Conserved Domains (2) summary

cd09601
Location:87 → 529

M1_APN_2; Peptidase M1 Aminopeptidase N family incudes tricorn interacting factor F3, Endoplasmic reticulum aminopeptidase 1 (ERAP1), Aminopeptidase Q (APQ)

pfam11838
Location:602 → 927

ERAP1_C; ERAP1-like C-terminal domain