lgg-1 Protein lgg-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: lgg-1
Official Full Name: Protein lgg-1
Primary source: WormBase:WBGene00002980
Locus tag: CELE_C32D5.9
See related: AllianceGenome:WB:WBGene00002980
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable GABA receptor binding activity; phosphatidylethanolamine binding activity; and ubiquitin protein ligase binding activity. Involved in several processes, including defense response to other organism; plasma membrane repair; and positive regulation of autophagosome assembly. Located in bounding membrane of organelle; neuron projection; and neuronal cell body. Is expressed in several structures, including egg-laying apparatus; germ cell; hypodermis; intestine; and nervous system. Orthologous to several human genes including GABARAP (GABA type A receptor-associated protein). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See lgg-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 2

Sequence:: Chromosome: II; NC_003280.10 (6346960..6347719, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans.
Title: Membrane localization of LGG-1/GABARAP is dispensable for autophagy in C. elegans.
A functional complementation of an lgg-1 null mutant with human GABARAP, its closer homolog showed that it localizes to autophagosomes and can rescue LGG-1 functions in the early embryo.
Title: Human GABARAP can restore autophagosome biogenesis in a C. elegans lgg-1 mutant.
LGG-1 is involved in the exposure of the 'eat-me signal' phosphatidylserine at the surface of the apoptotic cell to allow its recognition by the phagocytic cell, whereas LGG-2 is involved in later steps of phagocytosis to allow efficient cell corpse clearance by mediating the maturation/degradation of the phagosome.
Title: Autophagy mediates phosphatidylserine exposure and phagosome degradation during apoptosis through specific functions of GABARAP/LGG-1 and LC3/LGG-2.
While the molecular basis for the accumulation of lipidated and nonlipidated LGG-1 in atg-18 mutants remains unclear, starvation-induced increases in the nonlipidated form suggest that C. elegans boosts levels of lipidated LGG-1 by supplying more nonlipidated substrate, rather than by rendering phosphatidylethanolamine conjugation more efficient.
Title: Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans.
This work analyzed in apoptotic cell and phagocytic cell the localization of the two homologs of LC3/GABARAP proteins, namely, LGG-1 and LGG-2.
Title: Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo.
Data show the N-terminal of LGG-1 and LGG-2 adopts the closed and open form, respectively. They have distinct binding pockets that prefer different residues, influencing binding specificity and differential interaction with autophagy proteins.
Title: Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy.
During allophagy LGG-1 acts upstream of LGG-2 to allow its localization to autophagosomes.
Title: The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39.
both LGG-2 and LGG-1 act synergistically and are involved in dauer formation and longevity of the worm.
Title: The autophagosomal protein LGG-2 acts synergistically with LGG-1 in dauer formation and longevity in C. elegans.
Inactivation of unc-51, bec-1 and lgg-1, the worm counterparts of the yeast autophagy genes Atg1, Atg6 and Atg8 respectively, partially suppresses degeneration of neurons with toxic ion channel variants.
Title: Influence of autophagy genes on ion-channel-dependent neuronal degeneration in Caenorhabditis elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C32D5.9 (e-PCR)
- UniSTS:315657

STS2-147 (e-PCR)
- UniSTS:241815

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GABA receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylethanolamine binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagosome maturation	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to nitrogen starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to toxic substance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dauer larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in defense response to Gram-positive bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in determination of adult lifespan	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in necroptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in plasma membrane repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in programmed cell death	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in xenophagy	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
located_in autophagosome	IEA Inferred from Electronic Annotation more info
is_active_in autophagosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in autophagosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in autophagosome membrane	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in dendrite	IEA Inferred from Electronic Annotation more info
located_in lysosomal lumen	IEA Inferred from Electronic Annotation more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in perikaryon	IEA Inferred from Electronic Annotation more info
located_in phagocytic vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in phagophore assembly site	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Protein lgg-1

NP_495277.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

6346960..6347719 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_062876.8 → NP_495277.1 Protein lgg-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495277.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q09490

Conserved Domains (1) summary

cd01611
Location:5 → 116

GABARAP; Ubiquitin domain of GABA-receptor-associated protein